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Vimentin Human Protein

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SKU
BA1017S
Vimentin human recombinant protein, 0.1 mg
Size:
100 ug 250 ug
  $675.00
2 Weeks*
Bulk/Customize

Other products for "VIM or NM_003380"

Specifications
Specifications
Product Data
Species Human
Expression Host E. coli
Predicted MW 54 kDa
Concentration 1.0 mg/ml (after reconstitution)
Purity >95% pure determined by SDS Gel Electrophoresis
Presentation State Purified
Buffer Presentation State: Purified
State: Lyophilized protein
Buffer System: Final solution contains 30 mM Tris/HCI pH 8, 9.5M Urea, 2 mM DTT, 2 mM EDTA, 10 mM Methylammonium Chloride
Reconstitution Method Restore with 70 µl distilled water (final volume 100 µl).
Preparation Lyophilized protein
Recommended Dilution Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above), protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl, 2mM dithiothreitol, 10mM Tris-HCl, pH 7.4).
For immunization purposes, the solution can be further dialyzed against PBS (Phosphate Buffered Saline, e.g. Dulbecco's PBS).
Protein Description Standard Recombinant Human Vimentin.
Note Isoelectric Point: pI 5.3
Storage Store at 2-8°C (lyophilized) or at -20°C (reconstituted).
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Shipping Ambient
Reference Data
RefSeq NP_003371
Locus ID 7431
UniProt ID P08670
Cytogenetics 10p13
Synonyms VIM
Summary This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. provided by RefSeq, Aug 2017
Protein Categories ADC Targets, Intracellular Proteins, Nervous system Diseases
Protein Families 10mM Tris-HCl, 2mM dithiothreitol, e.g. Dulbecco's PBS)., pH 7.4).
For immunization purposes, protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl, the solution can be further dialyzed against PBS (Phosphate Buffered Saline
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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