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Cytokeratin 19 Human Protein

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SKU
BA1016
Cytokeratin 19 human recombinant protein, 0.25 mg
Size:
250 ug 100 ug
  $685.00
2 Weeks*
Bulk/Customize

Other products for "KRT19 or NM_002276"

Specifications
Specifications
Product Data
Species Human
Expression Host E. coli
Predicted MW 44 kDa
Concentration lot specific
Purity >95% determined by SDS gelelectrophoresis
Presentation State Purified
Buffer Presentation State: Purified
State: Lyophilized purified protein
Reconstitution Method BA1016: Restore with 175 µl distilled water (final volume 250 μl).
BA1016S: Restore with 70 µl distilled water (final volume 100 µl).
Final Solution: 30mM Tris/HCI pH 8, 9.5M Urea, 2mM DTT, 2mM EDTA, 10mM Methylammonium Chloride.
Preparation Lyophilized purified protein
Recommended Dilution Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4).
For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbeccos PBS).
Protein Description Recombinant Human keratin K19 (formerly also designated Cytokeratin 19).
Note

Isoelectric Point: pI 5.1
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Shipping Ambient
Reference Data
RefSeq NP_002267
Locus ID 3880
UniProt ID P08727
Cytogenetics 17q21.2
Synonyms Cytokeratin-19, Keratin-19, KRT19, CK19, K19
Summary The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. provided by RefSeq, Jul 2008
Protein Categories Intracellular Proteins
Protein Families 10 mM Tris-HCl, 2 mM dithiothreitol, both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, e.g. Dulbeccos PBS)., pH 7.4).
For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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