MED27 (1-311, His-Tag) Human Protein
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | E. coli |
| Expression cDNA Clone or AA Sequence | |
| Tag | His-Tag |
| Predicted MW | 37.8 kDa (334aa) |
| Concentration | 0.5mg/ml (determined by Bradford assay) |
| Purity | > 80% by SDS-PAGE |
| Presentation State | Purified |
| Buffer | Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol |
| Preparation | Liquid purified protein |
| Recommended Dilution | SDS-PAGE, Denatured |
| Protein Description | Recombinant human MED27 protein, fused to His-tag at N-terminus, was expressed in E.coli. |
| Storage | Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Reference Data | |
| RefSeq | NP_004260.2 |
| Locus ID | 9442 |
| UniProt ID | Q6P2C8 |
| Cytogenetics | 9q34.13 |
| Synonyms | Mediator complex subunit 27, CRAP34, CRSP34, CRSP8, TRAP37 |
| Summary | The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5. provided by RefSeq, Dec 2011 |
| Protein Categories | Intracellular Proteins |
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