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EEF1A1 / LENG7 (1-462) Human Protein

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SKU
AR51433PU-N
Recombinant human EF-1 alpha1/EEF1A1 protein
Size:
500 ug 100 ug
  $1,135.00
3 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host E. coli
Expression cDNA Clone or AA Sequence
Protein Sequence
MGKEKTHINI VVIGHVDSGK STTTGHLIYK CGGIDKRTIE KFEKEAAEMG KGSFKYAWVL DKLKAERERG ITIDISLWKF ETSKYYVTII DAPGHRDFIK NMITGTSQAD CAVLIVAAGV GEFEAGISKN GQTREHALLA YTLGVKQLIV GVNKMDSTEP PYSQKRYEEI VKEVSTYIKK IGYNPDTVAF VPISGWNGDN MLEPSANMPW FKGWKVTRKD GNASGTTLLE ALDCILPPTR PTDKPLRLPL QDVYKIGGIG TVPVGRVETG VLKPGMVVTF APVNVTTEVK SVEMHHEALS EALPGDNVGF NVKNVSVKDV RRGNVAGDSK NDPPMEAAGF TAQVIILNHP GQISAGYAPV LDCHTAHIAC KFAELKEKID RRSGKKLEDG PKFLKSGDAA IVDMVPGKPM CVESFSDYPP LGRFAVRDMR QTVAVGVIKA VDKKAAGAGK ITKSAQKAQK AK
Predicted MW 50.1 kDa (462aa)
Concentration 1mg/ml (determined by Bradford assay)
Purity > 90% by SDS-PAGE
Presentation State Purified
Buffer Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol
Preparation Liquid purified protein
Recommended Dilution SDS-PAGE, Denatured
Protein Description Recombinant human EEF1A1 protein is expressed in E.coli.
Storage Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Reference Data
RefSeq NP_001393.1
Locus ID 1915
UniProt ID P68104
Cytogenetics 6q13
Synonyms Eukaryotic translation elongation factor 1 alpha 1, Leukocyte receptor cluster (LRC) member 7, Elongation factor 1-alpha 1, EF-1-alpha-1, Elongation factor Tu, EF-Tu, Eukaryotic elongation factor 1 A-1, eEF1A-1, EEF1A, EF1A, LENG7, EE1A1, EF1A1, EF1alpha1
Summary This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. provided by RefSeq, Jul 2008
Protein Categories Growth Factors, Intracellular Proteins
Protein Families Druggable Genome
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Documents
Resources

file-alt Citations

Cytosolic PINK1 orchestrates protein translation during proteasomal stress by phosphorylating the translation elongation factor eEF1A1
Qin, Ye, Zheng et al
FEBS Lett (2020)
Cited by 16
AI Summary: OriGene products contributed to this study by providing human eEF1A1 and eEF1A2 cDNA clones, which were used to investigate the role of eEF1A1 in PINK1-mediated regulation of protein synthesis during proteasomal stress.
Snippet: site-containing 5' primer and Xho I site-containing 3' primer, and cloned into pET28a vector (Novagen) to generate His-tcPINK1 expression constructs. Human eEF1A1 and eEF1A2 cDNA clone was obtained from Origene. Full-length eEF1A1 ORF and
Combining laser capture microdissection and proteomics reveals an active translation machinery controlling invadosome formation
Ezzoukhry, Henriet, Cordelières et al
Nat Commun (2018) 9 (1), 2031 DOI: 10.1038/s41467-018-04461-9
AI Summary: OriGene products Caprin-myc, eIF3L-myc, HA-eEF1A1, and eEF2-GFP plasmids were used for transfection experiments to investigate the role of specific translational machinery components in invadosome formation.
Snippet: …Transfection Caprin-myc, eIF3L-myc, HA-eEF1A1, and eEF2-GFP were purchased form Origene. …Transfection Caprin-myc, eIF3L-myc, HA-eEF1A1, and eEF2-GFP were purchased form Origene. …
FigureFigureFigure
HIV-1 Uncoating and Reverse Transcription Require eEF1A Binding to Surface-Exposed Acidic Residues of the Reverse Transcriptase Thumb Domain
Rawle, Li, Swedberg et al
MBio (2018) 9 (2) DOI: 10.1128/mBio.00316-18
AI Summary: In this study, the OriGene recombinant eEF1A1 protein was used in binding assays to investigate its interaction with HIV-1 reverse transcriptase, helping to demonstrate the importance of specific RT thumb domain residues in viral uncoating and reverse transcription.
Snippet: …Purified recombinant eEF1A1 (OriGene) was biotinylated with EZ-Link-sulfo-NHS-biotin in accordance with the manufacturer’s instructions (Pierce Biotechnology, IL, USA) and immobilized on Octet Red system streptavidin-coated bio;... -0.5 Centrifugal Filter Unit with Ultracel-100 membrane (Amicon). …Purified recombinant eEF1A1 (OriGene) was biotinylated with EZ-Link-sulfo-NHS-biotin in accordance with the manufacturer’s instructions (Pierce Biotechnology, IL, USA) and immobilized on Octet Red system streptavidin-coated bio
FigureFigureFigure
Proteomic analyses of signalling complexes associated with receptor tyrosine kinase identify novel members of fibroblast growth factor receptor 3 interactome
Balek, Nemec, Konik et al
Cell. Signal. (2017)
AI Summary: In this study, OriGene products—specifically vectors expressing STAM1, SHOX2, and EEF1A1—were used to facilitate the identification and validation of novel protein interactions within the FGFR3 signalling complex.
Snippet: …Vectors expressing STAM1, SHOX2 and EEF1A1 were obtained from Origene (San Diego, CA). …Vectors expressing STAM1, SHOX2 and EEF1A1 were obtained from Origene (San Diego, CA). …
Expanded Polyalanine Tracts Function as Nuclear Export Signals and Promote Protein Mislocalization via Eukaryotic Translation Elongation Factor 1 Alpha 1
Li, Ng, Koon et al
J. Biol. Chem. (2017)
AI Summary: OriGene’s pCMV-XL5-eEF1A1 plasmid was used as a template to amplify the myc-eEF1A1 fragment for expression studies investigating the role of eEF1A1 in polyalanine protein mislocalization.
Snippet: The myc -eEF1A1 fragment was amplified by PCR using pCMV -XL5 -eEF1A1 (Origene) as template and then inserted into pcDNA3.1(+) vector using Eco RI and Xho I enzymes
ATXN2-AS, a gene antisense to ATXN2, is associated with spinocerebellar ataxia type 2 and amyotrophic lateral sclerosis
Li, Sun, Xia et al
Ann Neurol (2016) 80 (4), 600-15 DOI: 10.1002/ana.24761
Cited by 72
AI Summary: In this paper, OriGene's pCMV6-XL5-ATXN2 and pCMV6-XL5-ATXN2-AS expression plasmids were used to study the effects of ATXN2 and its antisense transcript (ATXN2-AS) in cellular models, enabling the authors to investigate the pathogenic role of ATXN2-AS in spinocerebellar ataxia type 2 and amyotrophic lateral sclerosis.
FigureFigure
Showing 1-6 of 11 papers.
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