AP1AR (1-302, His-tag) Human Protein

SKU AR51145PU-S

AP1AR (1-302, His-tag) human recombinant protein, 20 µg

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MGSSHHHHHH SSGLVPRGSH MGNCCWTQCF GLLRKEAGRL QRVGGGGGSK YFRTCSRGEH LTIEFENLVE SDEGESPGSS HRPLTEEEIV DLRERHYDSI AEKQKDLDKK IQKELALQEE KLRLEEEALY AAQREAARAA KQRKLLEQER QRIVQQYHPS NNGEYQSSGP EDDFESCLRN MKSQYEVFRS SRLSSDATVL TPNTESSCDL MTKTKSTSGN DDSTSLDLEW EDEEGMNRML PMRERSKTEE DILRAALKYS NKKTGSNPTS ASDDSNGLEW ENDFVSAEMD DNGNSEYSGF VNPVLELSDS GIRHSDTDQQ TR
• Tag: His-tag
• Predicted MW: 36.4 kDa
• Concentration: lot specific
• Purity: >90% by SDS - PAGE
• Presentation State: Purified
• Buffer: Presentation State: Purified; State: Liquid purified protein; Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1 mM DTT
• Preparation: Liquid purified protein
• Protein Description: Recombinant human AP1AR protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Storage: Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice

Reference Data

• RefSeq: NP_001121898
• Locus ID: 55435
• UniProt ID: Q63HQ0
• Cytogenetics: 4q25
• Synonyms: AP-1 complex-associated regulatory protein, C4orf16, 2c18, Adaptor-related protein complex 1-associated regulatory protein, Gamma-1-adaptin brefeldin A resistance protein, GBAR, Gamma-BAR, Gamma-A1-adaptin and kinesin interactor, Gadkin
• Summary: Necessary for adaptor protein complex 1 (AP-1)-dependent transport between the trans-Golgi network and endosomes. Regulates the membrane association of AP1G1/gamma1-adaptin, one of the subunits of the AP-1 adaptor complex. The direct interaction with AP1G1/gamma1-adaptin attenuates the release of the AP-1 complex from membranes. Regulates endosomal membrane traffic via association with AP-1 and KIF5B thus linking kinesin-based plus-end-directed microtubular transport to AP-1-dependent membrane traffic. May act as effector of AP-1 in calcium-induced endo-lysosome secretion. Inhibits Arp2/3 complex function; negatively regulates cell spreading, size and motility via intracellular sequestration of the Arp2/3 complex.[UniProtKB/Swiss-Prot Function]