PAF (1-111, His-Tag) Human Protein
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | E. coli |
| Expression cDNA Clone or AA Sequence | |
| Tag | His-Tag |
| Predicted MW | 14.4 kDa (134aa) confirmed by MALDI-TOF |
| Concentration | 0.5mg/ml (determined by Bradford assay) |
| Purity | > 90% by SDS-PAGE |
| Presentation State | Purified |
| Buffer | Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT |
| Preparation | Liquid purified protein |
| Recommended Dilution | SDS-PAGE |
| Protein Description | Recombinant human KIAA0101 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Storage | Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Reference Data | |
| RefSeq | NP_055551.1 |
| Locus ID | 9768 |
| UniProt ID | Q15004 |
| Cytogenetics | 15q22.31 |
| Synonyms | PCNA clamp associated factor, PAF15, PAF, p15(PAF), OEATC-1, OEATC, NS5ATP9, L5, KIAA0101 |
| Summary | PCNA-binding protein that acts as a regulator of DNA repair during DNA replication. Following DNA damage, the interaction with PCNA is disrupted, facilitating the interaction between monoubiquitinated PCNA and the translesion DNA synthesis DNA polymerase eta (POLH) at stalled replisomes, facilitating the bypass of replication-fork-blocking lesions. Also acts as a regulator of centrosome number.UniProtKB/Swiss-Prot Function |
| Protein Categories | Intracellular Proteins |
| Protein Families | Druggable Genome |
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