POLR2J2 (1-115, His-Tag) Human Protein

SKU
AR50776PU-N
Recombinant human RNA polymerase II subunit RPB11-b1/POLR2J2 protein
  $1,480.00
3 Weeks*
Bulk/Customize
Specifications
Specifications
Product Data
Species Human
Expression Host E. coli
Expression cDNA Clone or AA Sequence
Protein Sequence
MGSSHHHHHH SSGLVPRGSH MGSMNAPPAF ESFLLFEGEK ITINKDTKVP KACLFTINKE DHTLGNIIKS QLLKDPQVLF AGYKVPHPLE HKIIIRVQTT PDYSPQEAFT NAITDLISEL SLLEERFRTC LLPLRLLP
Tag His-Tag
Predicted MW 15.5 kDa (138aa) confirmed by MALDI-TOF
Concentration 0.25mg/ml (determined by Bradford assay)
Purity > 95% by SDS-PAGE
Presentation State Purified
Buffer Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT, 250mM Imidazole
Preparation Liquid purified protein
Recommended Dilution SDS-PAGE
Protein Description Recombinant human POLR2J2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Storage Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Reference Data
RefSeq NP_116581
Locus ID 246721
UniProt ID Q9GZM3
Cytogenetics 7q22.1
Synonyms DNA-directed RNA polymerase II subunit RPB11-b1, HRPB11B, RPB11b1
Summary This gene is a member of the RNA polymerase II subunit 11 gene family, which includes three genes in a cluster on chromosome 7q22.1 and a pseudogene on chromosome 7p13. The founding member of this family, DNA directed RNA polymerase II polypeptide J, has been shown to encode a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This locus produces multiple, alternatively spliced transcripts that potentially express isoforms with distinct C-termini compared to DNA directed RNA polymerase II polypeptide J. Most or all variants are spliced to include additional non-coding exons at the 3' end which makes them candidates for nonsense-mediated decay (NMD). Consequently, it is not known if this locus expresses a protein or proteins in vivo. provided by RefSeq, Jul 2008
Protein Categories Intracellular Proteins
Protein Families Transcription Factors
Protein Pathways Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
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