Hemoglobin alpha (1-142, His-Tag) Human Protein
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | E. coli |
| Expression cDNA Clone or AA Sequence | |
| Tag | His-Tag |
| Predicted MW | 19.5 kDa (179aa) |
| Concentration | 0.5mg/ml (determined by Bradford assay) |
| Purity | > 85% by SDS-PAGE |
| Presentation State | Purified |
| Buffer | Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol, 2M urea, 2mM DTT |
| Preparation | Liquid purified protein |
| Recommended Dilution | SDS-PAGE, Denatured |
| Protein Description | Recombinant human HBA2 protein, fused to His-tag at N-terminus, was expressed in E.coli. |
| Storage | Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Reference Data | |
| RefSeq | NP_000508.1 |
| Locus ID | 3039 |
| UniProt ID | P69905 |
| Cytogenetics | 16p13.3 |
| Synonyms | Hemoglobin subunit alpha, nucleophosmin/nucleoplasmin 2 |
| Summary | The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported. provided by RefSeq, Jul 2008 |
| Protein Categories | Cardiovascular diseases, Intracellular Proteins |
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