Streptavidin (25-183, His-Tag) Streptomyces, Streptomyces avidinii Protein

SKU
AR50021PU-N
Recombinant streptomyces avidinii Streptavidin protein
  $1,135.00
3 Weeks*
Bulk/Customize
Specifications
Specifications
Product Data
Species Streptomyces, Streptomyces avidinii
Expression Host E. coli
Expression cDNA Clone or AA Sequence
Protein Sequence
DP SKDSKAQVSA AEAGITGTWY NQLGSTFIVT AGADGALTGT YESAVGNAES RYVLTGRYDS APATDGSGTA LGWTVAWKNN YRNAHSATTW SGQYVGGAEA RINTQWLLTS GTTEANAWKS TLVGHDTFTK VKPSAASIDA AKKAGVNNGN PLDAVQQ
Tag His-Tag
Predicted MW 17.5 kDa (167aa) confirmed by MALDI-TOF
Concentration 1mg/ml (determined by BCA assay)
Purity > 95% by SDS-PAGE
Presentation State Purified
Buffer Liquid in. 20mM Tris-HCl buffer (pH 7.5)
Preparation Liquid purified protein
Recommended Dilution SDS-PAGE
Protein Description Recombinant His tagged streptavidin was expressed in E.coli and purified by using conventional chromatography techniques.
Storage Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Reference Data
RefSeq CAA27265.1
UniProt ID P22629
Synonyms Streptavidin, Streptomyces avidinii streptavidin, SA V1, SA V2, Streptavidin V1, Streptavidin V2.
Summary Streptavidin, a tetrameric protein secreted by Streptomyces avidinii, binds tightly to a small growth factor biotin. It finds wide use in molecular biology through its extraordinarily strong affinity for the vitamin biotin; the dissociation constant (Kd) of the biotin-streptavidin complex is on the order of ~10-15 mol/L. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. Recombinant His tagged streptavidin was expressed in E. coli and purified by using conventional chromatography techniques.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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