Leptin (22-167) Human Protein
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | E. coli |
| Expression cDNA Clone or AA Sequence | |
| Predicted MW | 16 kDa (147aa) confirmed by MALDI-TOF |
| Concentration | 1mg/ml (determined by Bradford assay) |
| Purity | > 95% by SDS-PAGE |
| Presentation State | Purified |
| Buffer | Liquid in. Phosphate-Buffered Saline (pH 7.4) |
| Endotoxin | < 1 EU per 1ug of protein (determined by LAL method) |
| Recommended Dilution | SDS-PAGE |
| Protein Description | Recombinant human leptin was overexpressed as insoluble protein aggregate in E.coli and purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. |
| Storage | Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Reference Data | |
| RefSeq | NP_000221 |
| Locus ID | 3952 |
| UniProt ID | P41159 |
| Cytogenetics | 7q32.1 |
| Synonyms | LEP, OB, OBS, Leptin, Obesity factor, Obese protein, Leptin, Leptin Murine Obesity Homolog, Leptin Precursor Obesity Factor, Obesity, Obesity homolog mouse, Obesity Murine Homolog Leptin |
| Summary | This gene encodes a protein that is secreted by white adipocytes into the circulation and plays a major role in the regulation of energy homeostasis. Circulating leptin binds to the leptin receptor in the brain, which activates downstream signaling pathways that inhibit feeding and promote energy expenditure. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis, reproduction, bone formation and wound healing. Mutations in this gene and its regulatory regions cause severe obesity and morbid obesity with hypogonadism in human patients. A mutation in this gene has also been linked to type 2 diabetes mellitus development. provided by RefSeq, Aug 2017 |
| Protein Categories | Cytokines, Endocrine and metabolic diseases, Growth Factors, Secreated Proteins |
| Protein Families | Druggable Genome, Secreted Protein |
| Protein Pathways | Adipocytokine signaling pathway, Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway, Neuroactive ligand-receptor interaction |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Citations
Human Herpesvirus 6A and 6B inhibit in vitro angiogenesis by induction of Human Leukocyte Antigen G
Rizzo, D'Accolti, Bortolotti et al
Sci Rep (2018) 8 (1), 17683 DOI: 10.1038/s41598-018-36146-0
Rizzo, D'Accolti, Bortolotti et al
Sci Rep (2018) 8 (1), 17683 DOI: 10.1038/s41598-018-36146-0
AI Summary: OriGene’s purified recombinant HLA-G protein was used to treat endothelial cells, enabling the researchers to assess the role of HLA-G in HHV-6-induced inhibition of angiogenesis.
Snippet: Article.;HUVECs were treated with 3 μg/ml of recombinant U94 protein, obtained and purified as described24, or with 1 μg/ml of purified recombinant HLA-G protein (Origene, Rockville, MD, USA)
Showing 1-1 of 1 papers.
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