SNAP25 (1-207) Human Protein
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | E. coli |
| Expression cDNA Clone or AA Sequence | |
| Predicted MW | 23 kDa (207aa) confirmed by MALDI-TOF |
| Concentration | 1mg/ml (determined by Bradford assay) |
| Purity | > 95% by SDS-PAGE |
| Presentation State | Purified |
| Buffer | Liquid in. 20mM Tris-HCl buffer (pH 7.5) containing 50mM NaCl, 5m MDTT, 1mM EDTA, 10%glycerol |
| Preparation | Liquid purified protein |
| Recommended Dilution | SDS-PAGE |
| Protein Description | SNAP-25 gene was amplified by PCR from C.elegans and cloned into an E. coli expression vector. SNAP-25 was purified by using conventional chromatography techniques. |
| Storage | Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Reference Data | |
| RefSeq | NP_505641.2 |
| UniProt ID | O62414 |
| Synonyms | Resistance to Inhibitors of Cholinesterase family member, SNAP25, SNAP-25, Synaptosomal-associated protein 25kDa, FLJ23079, RIC-4, RIC4, SEC9, SNAP 25, bA416N4.2, dJ1068F16.2, SuP, resistance to inhibitors of cholinesterase 4 homolog, super protein, synaptosomal-associated 25 kDa protein, synaptosomal-associated protein 25, RIC 4 |
| Summary | SNAP-25 is a presynaptic plasma membrane protein essential for synaptic vesicle fusion and neurotransmitter release. The membrane proteins SNAP-25, synaptobrevin and syntaxin make up the core complex (SNARE) essential for docking and holding synaptic vesicles at the pre synaptic membrane. SNAP-25 gene was amplified by PCR from C. elegans and cloned into an E. coli expression vector. SNAP-25 was purified by using conventional chromatography techniques. |
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