PPCS (1-311, His-Tag) Human Protein
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | E. coli |
| Expression cDNA Clone or AA Sequence | |
| Tag | His-Tag |
| Predicted MW | 36.1 kDa (331aa) confirmed by MALDI-TOF |
| Concentration | 1mg/ml (determined by Bradford assay) |
| Purity | > 95% by SDS-PAGE |
| Presentation State | Purified |
| Buffer | Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol |
| Preparation | Liquid purified protein |
| Recommended Dilution | SDS-PAGE |
| Protein Description | Recombinant human PPCS protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. |
| Storage | Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Reference Data | |
| RefSeq | NP_078940 |
| Locus ID | 79717 |
| UniProt ID | Q9HAB8 |
| Cytogenetics | 1p34.2 |
| Synonyms | Phosphopantothenate--cysteine ligase, COAB, PPC synthetase |
| Summary | Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCS (EC 6.3.2.5), one of the last enzymes in this pathway, converts phosphopantothenate to phosphopantothenoylcysteine (Daugherty et al., 2002 PubMed 11923312).supplied by OMIM, Mar 2008 |
| Protein Categories | Cardiovascular diseases, Enzyme: Ligase, Intracellular Proteins |
| Protein Pathways | Metabolic pathways, Pantothenate and CoA biosynthesis |
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