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RSPO1 Human Protein

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SKU
AR31177PU-N
RSPO1 human recombinant protein, 20 µg
Size:
20 ug
  $510.00
2 Weeks*
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Other products for "RSPO1 or NM_001038633"

Specifications
Specifications
Product Data
Species Human
Expression Host CHO
Expression cDNA Clone or AA Sequence
Protein Sequence
SRGIKGKRQR RISAEGSQAC AKGCELCSEV NGCLKCSPKL FILLERNDIR QVGVCLPSCP PGYFDARNPD MNKCIKCKIE HCEACFSHNF CTKCKEGLYL HKGRCYPACP EGSSAANGTM ECSSPAQCEM SEWSPWGPCS KKQQLCGFRR GSEERTRRVL HAPVGDHAAC SDTKETRRCT VRRVPCPEGQ KRRKGGQGRR ENANRNLARK ESKEAGAGSR RRKGQQQQQQ QGTVGPLTSA GPA
Predicted MW 26.3 kDa
Purity >95% by SDS-PAGE and HPLC
Presentation State Purified
Buffer Presentation State: Purified
State: Lyophilized purified protein from 10 mM Sodium Phosphate, pH 7, 5 + 150 mM NaCl
Stabilizer: None
Bioactivity R-spondin-1 enhances BMP-2 mediated differentiation of MC3T3-E1 cells. The expected ED50 is 1.0-3.0 ug/ml.
Reconstitution Method Restore in a Water to ac concentration of 0.1-1.0 mg/ml
Preparation Lyophilized purified protein
Protein Description Recombinant human R-Spondin-1 is a 26.7 kDa protein consisting of 243 amino acid residues.  Due to glycosylation, R-Spondin-1 migrates at an apparent molecular weight of approximately 40.0 kDa by SDS PAGE analysis under reducing conditions.
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Shipping Ambient
Reference Data
RefSeq NP_001033722
Locus ID 284654
UniProt ID Q2MKA7
Cytogenetics 1p34.3
Synonyms R-spondin-1
Summary This gene encodes a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. Alternative splicing results in multiple transcript variants. provided by RefSeq, Apr 2014
Protein Categories Intracellular Proteins, Secreated Proteins
Protein Families Secreted Protein
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Documents
Resources

file-alt Citations

PDGFRα + pericryptal stromal cells are the critical source of Wnts and RSPO3 for murine intestinal stem cells in vivo
Greicius, Kabiri, Sigmundsson et al
PNAS (2018)
AI Summary: OriGene products, specifically the Myc-tagged RSPO1 (RC217921) and RSPO3 (RC205016) expression plasmids, were used in this study to investigate and compare the potency of RSPO proteins in stimulating Wnt/β-catenin signaling and organoid growth.
Snippet: plates. STF3A cells were transfected 24 h post plating with total 600 ng of the indicated expression plasmids [2–200 ng Myc-tagged RSPO1 or RSPO3 (catalog nos. RC217921 and RC205016, respectively;OriGene), 200 ng pCS-mCherry as reference, 200–398 ng pPGK as empty vector, in Lipofectamine 2000 (catalog no. 11668019;Life Technology)]. Cells were harvested 16 h post transfection in PBS with 0
N-Glycosylation of Human R-Spondin 1 Is Required for Efficient Secretion and Stability but Not for Its Heparin Binding Ability
Chang, Hsu, Weng et al
Int J Mol Sci (2016) 17 (6) DOI: 10.3390/ijms17060937
Cited by 15
AI Summary: OriGene contributed to this study by providing the pCMV6-hRspo1-myc-ddk plasmid, which was used for expressing human Rspo1 in experiments investigating the role of N-glycosylation in Rspo1 secretion and stability.
Snippet: secretion. 4. Experimental Section. 4.1. Plasmid Construction and Site-Directed Mutagenesis. The vector expressing human Rspo1 with a myc-tag (pCMV6-hRspo1-myc-ddk) was purchased from Origene (Rockville, MD, USA). ... Cite SaveSaving
Protective role of R-spondin1, an intestinal stem cell growth factor, against radiation-induced gastrointestinal syndrome in mice
Bhanja, Saha, Kabarriti et al
PLoS One (2009) 4 (11), e8014 DOI: 10.1371/journal.pone.0008014
Cited by 127
AI Summary: In this study, OriGene products including anti-LGR5 antibody (TA322798) and anti-β-catenin antibody (TA301790) were used for immunohistochemical analysis to assess the effects of R-spondin1 on intestinal stem cell regeneration and Wnt/β-catenin pathway activation following radiation in mice.
FigureFigureFigure
Showing 1-3 of 3 papers.
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