Interleukin-12 alpha / IL12A Mouse Protein

SKU AR31173PU-N

Interleukin-12 alpha / IL12A mouse protein, 10 µg

Product Data

• Species: Mouse
• Expression Host: CHO
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  p35 Subunit: RVIPVSGPAR CLSQSRNLLK TTDDMVKTAR EKLKHYSCTA EDIDHEDITR DQTSTLKTCL PLELHKNESC LATRETSSTT RGSCLPPQKT SLMMTLCLGS IYEDLKMYQT EFQAINAALQ NHNHQQIILD KGMLVAIDEL MQSLNHNGET LRQKPPVGEA DPYRVKMKLC ILLHAFSTRV VTINRVMGYL SSA ; p40 Subunit: MWELEKDVYV VEVDWTPDAP GETVNLTCDT PEEDDITWTS DQRHGVIGSG KTLTITVKEF LDAGQYTCHK GGETLSHSHL LLHKKENGIW STEILKNFKN KTFLKCEAPN YSGRFTCSWL VQRNMDLKFN IKSSSSSPDS RAVTCGMASL SAEKVTLDQR DYEKYSVSCQ EDVTCPTAEE TLPIELALEA RQQNKYENYS TSFFIRDIIK PDPPKNLQMK PLKNSQVEVS WEYPDSWSTP HSYFSLKFFV RIQRKKEKMK ETEEGCNQKG AFLVEKTSTE VQCKGGNVCV QAQDRYYNSS CSKWACVPCR VRS;
• Predicted MW: 75 kDa
• Purity: >98% by SDS-PAGE and HPLC analyses
• Presentation State: Purified
• Buffer: Presentation State: Purified; State: Lyophilized purified protein; Stabilizer: None
• Bioactivity: Assay #1: The ED50 was determined by the stimulation of IFN-gamma production by murine splenocytes co-stimulated with IL-18 is < 0.1 ng/ml (> 1 x 107 units/mg). Assay #2: Determined by its ability to stimulate the proliferation of 2D6 cells. The expected ED50 is ≤0.1ng/ml (≥1 x 107 units/mg).
• Reconstitution Method: The lyophilized Murine IL-12 is soluble in most aqueous buffers. Restore in 1 x PBS pH 7.2-7.4 to a concentration of 0.1 - 1.0 mg/ml. This solution can be stored at -20°C for future use.
• Preparation: Lyophilized purified protein
• Protein Description: Murine IL-12 is a disulfide linked heterodimeric protein with a molecular weight of 70 kDa comprised of disulfide-bonded 35 kDa (p35) and 40kDa (p40) subunits. AA sequence: p35 Subunit: RVIPVSGPAR CLSQSRNLLK TTDDMVKTAR EKLKHYSCTA EDIDHEDITR DQTSTLKTCL PLELHKNESC LATRETSSTT RGSCLPPQKT SLMMTLCLGS IYEDLKMYQT EFQAINAALQ NHNHQQIILD KGMLVAIDEL MQSLNHNGET LRQKPPVGEA DPYRVKMKLC ILLHAFSTRV VTINRVMGYL SSA p40 Subunit: MWELEKDVYV VEVDWTPDAP GETVNLTCDT PEEDDITWTS DQRHGVIGSG KTLTITVKEF LDAGQYTCHK GGETLSHSHL LLHKKENGIW STEILKNFKN KTFLKCEAPN YSGRFTCSWL VQRNMDLKFN IKSSSSSPDS RAVTCGMASL SAEKVTLDQR DYEKYSVSCQ EDVTCPTAEE TLPIELALEA RQQNKYENYS TSFFIRDIIK PDPPKNLQMK PLKNSQVEVS WEYPDSWSTP HSYFSLKFFV RIQRKKEKMK ETEEGCNQKG AFLVEKTSTE VQCKGGNVCV QAQDRYYNSS CSKWACVPCR VRS Endotoxin Levels: < 0.1 ng/µg of protein (<1EU/µg)
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.; After reconstitution store the antibody undiluted at 2-8°C for one month ; or (in aliquots) at -20°C long term.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Ambient

Reference Data

• RefSeq: NP_001152896
• Locus ID: 16159
• UniProt ID: P43431
• Cytogenetics: 3 31.92 cM
• Synonyms: IL-12A, IL-12 subunit p35, NKSF1, CLMF p35
• Summary: Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.[UniProtKB/Swiss-Prot Function]