CNTF Rat Protein

CAT#: AR31054PU-N

CNTF rat recombinant protein, 5 µg

Product Images

Specifications

Product Data

• Species: Rat
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MAFAEQTPLT LHRRDLCSRS IWLARKIRSD LTALMESYVK HQGLNKNINL DSVDGVPVAS TDRWSEMTEA ERLQENLQAY RTFQGMLTKL LEDQRVHFTP TEGDFHQAIH TLMLQVSAFA YQLEELMLLE QKIPENEADG MPATVGDGGL FEKKLWGLKV LQELSQWTVR SIHDLRVISS HQMGISALES HYGAKDKQMA FAEQTPLTLH RRDLCSRSIW LARKIRSDLT ALMESYVKHQ GLNKNINLDS VDGVVASTDR WSEMTEAERL QENLQAYRTF QGMLTKLLED QRVHFTPTEG DFHQAIHTLM LQVSAFAYQL EELMVLLEQK IPENEADGMP ATVGDGGLFE KKLWGLKVLQ ELSQWTVRSI HDLRVISSHQ MISALESHYG AKDKQM
  Result by N-terminal sequencing: AFAEQTPLTL HR
• Predicted MW: 22.7 kDa
• Purity: >98% by SDS-PAGE and Silver stain
• Presentation: Purified
• Buffer: Presentation State: Purified
  State: Lyophilized purified protein
  Buffer System: 5 mM Sodium Acetate, pH 6.5
  Stabilizer: None
• Bioactivity: Biological: Measured in a cell proliferation assay using TF1 human erythroleukemic cells [Kitamura T et al, J Cell Physiol 140, 1989). The ED50 for this effect is typically 3 - 15 ng/ml.
• Endotoxin: < 0.1ng/µg of CNTF
• Reconstitution: Restore in PBS to a concentration of 0.1 mg/ml.
  This solution can be diluted water or other buffered solutions or stored at -20°C for future use.
• Preparation: Lyophilized purified protein
• Protein Description: Recombinant Rat Ciliary Neurotrophic Factor.
  The cDNA for recombinant Rat CNTF (Ala2–Met200) was cloned from total RNA of a Rat embryo using standard protocols.
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_037298
• Locus ID: 25707
• UniProt ID: P20294
• Cytogenetics: 1q43
• Summary: a cytokine involved in neuronal degeneration and adipocyte gene expression [RGD, Feb 2006]