CNTF Rat Protein

SKU
AR31054PU-L
CNTF rat recombinant protein, 25 µg
  $415.00
2 Weeks*
Bulk/Customize
Specifications
Specifications
Product Data
Species Rat
Expression Host E. coli
Expression cDNA Clone or AA Sequence
Protein Sequence
MAFAEQTPLT LHRRDLCSRS IWLARKIRSD LTALMESYVK HQGLNKNINL DSVDGVPVAS TDRWSEMTEA ERLQENLQAY RTFQGMLTKL LEDQRVHFTP TEGDFHQAIH TLMLQVSAFA YQLEELMLLE QKIPENEADG MPATVGDGGL FEKKLWGLKV LQELSQWTVR SIHDLRVISS HQMGISALES HYGAKDKQMA FAEQTPLTLH RRDLCSRSIW LARKIRSDLT ALMESYVKHQ GLNKNINLDS VDGVVASTDR WSEMTEAERL QENLQAYRTF QGMLTKLLED QRVHFTPTEG DFHQAIHTLM LQVSAFAYQL EELMVLLEQK IPENEADGMP ATVGDGGLFE KKLWGLKVLQ ELSQWTVRSI HDLRVISSHQ MISALESHYG AKDKQM
Result by N-terminal sequencing: AFAEQTPLTL HR
Predicted MW 22.7 kDa
Purity >98% by SDS-PAGE and Silver stain
Presentation State Purified
Buffer Presentation State: Purified
State: Lyophilized purified protein
Buffer System: 5 mM Sodium Acetate, pH 6.5
Stabilizer: None
Bioactivity Biological: Measured in a cell proliferation assay using TF1 human erythroleukemic cells [Kitamura T et al, J Cell Physiol 140, 1989). The ED50 for this effect is typically 3 - 15 ng/ml.
Endotoxin < 0.1ng/µg of CNTF
Reconstitution Method Restore in PBS to a concentration of 0.1 mg/ml.
This solution can be diluted water or other buffered solutions or stored at -20°C for future use.
Preparation Lyophilized purified protein
Protein Description Recombinant Rat Ciliary Neurotrophic Factor.
The cDNA for recombinant Rat CNTF (Ala2–Met200) was cloned from total RNA of a Rat embryo using standard protocols.
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Shipping Ambient
Reference Data
RefSeq NP_037298
Locus ID 25707
UniProt ID P20294
Cytogenetics 1q43
Synonyms Ciliary Neurotrophic Factor
Summary a cytokine involved in neuronal degeneration and adipocyte gene expression RGD, Feb 2006
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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