CNTF Rat Protein
Specifications
| Product Data | |
| Species | Rat |
|---|---|
| Expression Host | E. coli |
| Expression cDNA Clone or AA Sequence | |
| Predicted MW | 22.7 kDa |
| Purity | >98% by SDS-PAGE and Silver stain |
| Presentation State | Purified |
| Buffer | Presentation State: Purified State: Lyophilized purified protein Buffer System: 5 mM Sodium Acetate, pH 6.5 Stabilizer: None |
| Bioactivity | Biological: Measured in a cell proliferation assay using TF1 human erythroleukemic cells [Kitamura T et al, J Cell Physiol 140, 1989). The ED50 for this effect is typically 3 - 15 ng/ml. |
| Endotoxin | < 0.1ng/µg of CNTF |
| Reconstitution Method | Restore in PBS to a concentration of 0.1 mg/ml. This solution can be diluted water or other buffered solutions or stored at -20°C for future use. |
| Preparation | Lyophilized purified protein |
| Protein Description | Recombinant Rat Ciliary Neurotrophic Factor. The cDNA for recombinant Rat CNTF (Ala2–Met200) was cloned from total RNA of a Rat embryo using standard protocols. |
| Storage | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NP_037298 |
| Locus ID | 25707 |
| UniProt ID | P20294 |
| Cytogenetics | 1q43 |
| Synonyms | Ciliary Neurotrophic Factor |
| Summary | a cytokine involved in neuronal degeneration and adipocyte gene expression RGD, Feb 2006 |
Reviews
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Resources
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