PYCR1 (1-319, His-tag) Human Protein

CAT#: AR09751PU-N

PYCR1 (1-319, His-tag) human recombinant protein, 50 µg

Product Images

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MGSSHHHHHH SSGLVPRGSH MSVGFIGAGQ LAFALAKGFT AAGVLAAHKI MASSPDMDLA TVSALRKMGV KLTPHNKETV QHSDVLFLAV KPHIIPFILD EIGADIEDRH IVVSCAAGVT ISSIEKKLSA FRPAPRVIRC MTNTPVVVRE GATVYATGTH AQVEDGRLME QLLSSVGFCT EVEEDLIDAV TGLSGSGPAY AFTALDALAD GGVKMGLPRR LAVRLGAQAL LGAAKMLLHS EQHPGQLKDN VSSPGGATIH ALHVLESGGF RSLLINAVEA SCIRTRELQS MADQEQVSPA AIKKTILDKV KLDSPAGTAL SPSGHTKLLP RSLAPAGKD
• Tag: His-tag
• Predicted MW: 35.5 kDa
• Concentration: lot specific
• Purity: >90%
• Presentation: Purified
• Buffer: Presentation State: Purified
  State: Liquid purified protein
  Buffer System: 20 mM Tris-HCl Buffer (pH 8.5) containing 10% Glycerol
• Bioactivity: Specific: > 0.03 unit/mg.
  One unit will oxidize 1.0 umole of L-proline to 1-pyrroline-5-carboxylate per minute in the presence of beta NAD at pH 10.0 at 25°C.
  (Activity assay see "Protocols").
• Preparation: Liquid purified protein
• Applications: Protocol:
  Biological Assay:
  1. Prepare an assay buffer with the following concentrations:
  200 mM Tris-HCl (pH10.0) and 10 mM NAD, 20 mM L-proline and load 150 ul into each well.
  2. Add 50 ul of 1.25 ug and 2.5 ug recombinant PYCR1 protein to each well.
  3. Record the increase in A340nm for 5 minutes at 25°C.
• Protein Description: Recombinant human PYCR1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_001269208
• Locus ID: 5831
• UniProt ID: E2QRB3
• Cytogenetics: 17q25.3
• Synonyms: ARCL2B; ARCL3B; P5C; P5CR; PIG45; PP222; PRO3; PYCR
• Summary: This gene encodes an enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. The protein forms a homopolymer and localizes to the mitochondrion. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]
• Protein Pathways: Arginine and proline metabolism, Metabolic pathways