PYCR1 (1-319, His-Tag) Human Protein
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | E. coli |
| Expression cDNA Clone or AA Sequence | |
| Tag | His-Tag |
| Predicted MW | 35.5 kDa (339aa) confirmed by MALDI-TOF |
| Concentration | 0.5mg/ml (determined by Bradford assay) |
| Purity | > 90% by SDS-PAGE |
| Presentation State | Purified |
| Buffer | Liquid in. 20mM Tris-HCl buffer (pH 8.5) containing 10% glycerol |
| Bioactivity | Specific activity is > 0.03 unit/mg. One unit will oxidize 1.0 umole of L-proline to 1-pyrroline-5-carboxylate per minute in the presence of beta NAD at pH 10.0 at 25C. |
| Preparation | Liquid purified protein |
| Recommended Dilution | SDS-PAGE, Enzyme Activity |
| Protein Description | Recombinant human PYCR1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. |
| Storage | Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Reference Data | |
| RefSeq | NP_008838 |
| Locus ID | 5831 |
| UniProt ID | P32322 |
| Cytogenetics | 17q25.3 |
| Synonyms | Pyrroline-5-carboxylate reductase 1, ARCL2B, P5C, P5CR, PIG45, PP222, PRO3 |
| Summary | This gene encodes an enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. The protein forms a homopolymer and localizes to the mitochondrion. Alternative splicing results in multiple transcript variants. provided by RefSeq, Aug 2013 |
| Protein Categories | Intracellular Proteins |
| Protein Pathways | Arginine and proline metabolism, Metabolic pathways |
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