OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

DUT (70-252, His-Tag) Human Protein

Be the first to review this product
SKU
AR09663PU-L
Recombinant human DUTPase/DUT protein
Size:
500 ug
  $1,135.00
3 Weeks*
Bulk/Customize

Other products for "DUT or NM_001025248"

Specifications
Specifications
Product Data
Species Human
Expression Host E. coli
Expression cDNA Clone or AA Sequence
Protein Sequence
MGSSHHHHHH SSGLVPRGSH MASTVGAAGW KGELPKAGGS PAPGPETPAI SPSKRARPAE VGGMQLRFAR LSEHATAPTR GSARAAGYDL YSAYDYTIPP MEKAVVKTDI QIALPSGCYG RVAPRSGLAA KHFIDVGAGV IDEDYRGNVG VVLFNFGKEK FEVKKGDRIA QLICERIFYP EIEEVQALDD TERGSGGFGS TGKN
Tag His-Tag
Predicted MW 21.6 kDa (204aa) confirmed by MALDI-TOF
Concentration 1mg/ml (determined by Bradford assay)
Purity > 90% by SDS-PAGE
Presentation State Purified
Buffer Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl.
Preparation Liquid purified protein
Recommended Dilution SDS-PAGE
Protein Description Recombinant human DUT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Storage Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Reference Data
RefSeq NP_001020419
Locus ID 1854
UniProt ID P33316
Cytogenetics 15q21.1
Synonyms FLJ20622, duTPase, duTP pyrophosphatase, duTP diphosphatase, Deoxyuridine Triphosphatase, Deoxyuridine 5-triphosphate nucleotidohydrolase mitochondrial
Summary This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19. provided by RefSeq, Jul 2008
Protein Categories Intracellular Proteins
Protein Families Druggable Genome
Protein Pathways Metabolic pathways, Pyrimidine metabolism
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products