PPIL2 (1-527, His-Tag) Human Protein

SKU
AR09397PU-N
Recombinant human Cyclophilin-like 2/PPIL2 protein
  $570.00
3 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host E. coli
Expression cDNA Clone or AA Sequence
Protein Sequence
MGKRQHQKDK MYITCAEYTH FYGGKKPDLP QTNFRRLPFD HCSLSLQPFV YPVCTPDGIV FDLLNIVPWL KKYGTNPSNG EKLDGRSLIK LNFSKNSEGK YHCPVLFTVF TNNTHIVAVR TTGNVYAYEA VEQLNIKAKN FRDLLTDEPF SRQDIITLQD PTNLDKFNVS NFYHVKNNMK IIDPDEEKAK QDPSYYLKNT NAETRETLQE LYKEFKGDEI LAATMKAPEK KKVDKLNAAH YSTGKVSASF TSTAMVPETT HEAAAIDEDV LRYQFVKKKG YVRLHTNKGD LNLELHCDLT PKTCENFIRL CKKHYYDGTI FHRSIRNFVI QGGDPTGTGT GGESYWGKPF KDEFRPNLSH TGRGILSMAN SGPNSNRSQF FITFRSCAYL DKKHTIFGRV VGGFDVLTAM ENVESDPKTD RPKEEIRIDA TTVFVDPYEE ADAQIAQERK TQLKVAPETK VKSSQPQAGS QGPQTFRQGV GKYINPAATE QQRKSPQPVP LSPCPRRSPV GVLGTSAPGS SRLPDDH
Tag His-Tag
Predicted MW 61.6 kDa (547aa)
Concentration 0.5mg/ml (determined by Bradford assay)
Purity > 90% by SDS-PAGE
Presentation State Purified
Buffer Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl
Bioactivity Specific activity is > 290nmol/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAPF-pNA per minute at 37C in Tris-Hcl pH8.0 using chymotrypsin..
Preparation Liquid purified protein
Recommended Dilution SDS-PAGE, Enzyme Activity
Protein Description Recombinant PPIL2 protein, fused to His-tag, was expressed in E.coli and purified by using conventional chromatography techniques.
Storage Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Reference Data
RefSeq NP_680481.1
Locus ID 23759
UniProt ID Q13356
Cytogenetics 22q11.21
Synonyms UBOX7, U-box domain containing 7, RING-type E3 ubiquitin-protein ligase PPIL2, PPIase, Peptidylprolyl isomerase like 2, Peptidyl-prolyl cis-trans isomerase-like 2, hCyP-60, Cyp60, Cyclophilin-like protein Cyp-60, Cyclophilin-60, CYC4
Summary This gene is a member of the cyclophilin family of peptidylprolyl isomerases. The cyclophilins are a highly conserved ubiquitous family, members of which play an important role in protein folding, immunosuppression by cyclosporin A, and infection of HIV-1 virions. This protein interacts with the proteinase inhibitor eglin c and is localized in the nucleus. Alternatively spliced transcript variants have been found for this gene. provided by RefSeq, Dec 2015
Protein Categories Intracellular Proteins
Protein Families pH 8.0, Protocol: Activity Assay
1. Prepare 170 ul assay buffer into a suitable container and pre-chill on ice before use:
The final concentrations are 200 mM Tris-HCl
Protein Pathways Ubiquitin mediated proteolysis
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