Galectin-9 (1-148, His-tag) Human Protein

CAT#: AR09380PU-N

Galectin-9 (1-148, His-tag) human recombinant protein, 50 µg

Product Images

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MGSSHHHHHH SSGLVPRGSH MAFSGSQAPY LSPAVPFSGT IQGGLQDGLQ ITVNGTVLSS SGTRFAVNFQ TGFSGNDIAF HFNPRFEDGG YVVCNTRQNG SWGPEERKTH MPFQKGMPFD LCFLVQSSDF KVMVNGILFV QYFHRVPFHR VDTISVNGSV QLSYISFQ
• Tag: His-tag
• Predicted MW: 18.5 kDa
• Concentration: lot specific
• Purity: >90% by SDS - PAGE
• Presentation: Purified
• Buffer: Presentation State: Purified
  State: Liquid purified protein
  Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1 M NaCl
• Bioactivity: Biological:
  The ED50 for this effect is is 1.25–5 ug/ml, measured by its ability to agglutinate human red blood cells.
  (Biological assay see "Protocols").
• Preparation: Liquid purified protein
• Applications: Protocol: Biological Assay :
  1. Mix equal volumes of human blood and Alsever’s solution (pH 7.0). (Alsever’s solution: NaCl 0.42 g, Sodium citric acid 0.8g, Citric acid 0.055 g, D - glucose 2.05g in DW100 ml)
  2. Centrifuge at 15000rpm for 10 minutes and wash four times with PBS.
  3. Dilute packed cells in a 0.5 mg/ml trypsin - EDTA solution to give 4% red cell suspension.
  4. Incubate for 1h at 37°C and wash four times with PBS.
  5 . Dilute packed cells in PBS to give 4% red cell suspension.
  6 . Load 50ul of 0.5%BSA - in - 0.15M - NaCl solution and 25ul of 4% - Red Cell - in - PBS in u shaped wells.
  7 . Add 25ul of serial diluted Galectin protein in PBS to each well plate. (Round bottom 96 well plate)
  8 . Incubate for 30min at room temperature to observe visible agglutination.
• Protein Description: Recombinant Galectin-9 protein, fused to His-tag, was expressed in E.coli and purified by using conventional chromatography techniques.
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_001317092
• Locus ID: 3965
• UniProt ID: O00182
• Cytogenetics: 17q11.2
• Synonyms: HUAT; LGALS9A
• Summary: The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The protein encoded by this gene is an S-type lectin. It is overexpressed in Hodgkin's disease tissue and might participate in the interaction between the H&RS cells with their surrounding cells and might thus play a role in the pathogenesis of this disease and/or its associated immunodeficiency. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008]