Cytokeratin 20 Human Protein

CAT#: AR09365PU-S

Cytokeratin 20 human recombinant protein, 0.1 mg

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Predicted MW: 46 kDa
• Concentration: lot specific
• Purity: >95% determined by SDS gelelectrophoresis
• Presentation: Purified
• Buffer: Presentation State: Purified
  State: Lyophilised purified protein
  Buffer System: 30 mM Tris/HCI pH 8, 9.5 M urea, 2 mM DTT, 2 mM EDTA, 10 mM methylammonium chloride
• Reconstitution: Reconstitute with 175 µl distilled water (final volume 250 µl)
  Reconstitute with 70 µl distilled water (final volume 100 µl)
• Preparation: Lyophilised purified protein
• Applications: Protein standard in 1D and 2D SDS gelelectrophoresis.
  Immunoassays.
  Immunization.
  Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).
• Protein Description: Recombinant human keratin K20 (formerly also designated cytokeratin 20).
• Note: Isoelectric Point pI 5.66
• Storage: Prior to reconstitution store at 2-8°C.
  Following reconstitution store the antibody at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_061883
• Locus ID: 54474
• UniProt ID: P35900
• Cytogenetics: 17q21.2
• Synonyms: CD20; CK-20; CK20; K20; KRT21
• Summary: The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008]