Cytokeratin 20 Human Protein
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | E. coli |
| Predicted MW | 46 kDa |
| Concentration | lot specific |
| Purity | >95% determined by SDS gelelectrophoresis |
| Presentation State | Purified |
| Buffer | Presentation State: Purified State: Lyophilised purified protein Buffer System: 30 mM Tris/HCI pH 8, 9.5 M urea, 2 mM DTT, 2 mM EDTA, 10 mM methylammonium chloride |
| Reconstitution Method | Reconstitute with 175 µl distilled water (final volume 250 µl) Reconstitute with 70 µl distilled water (final volume 100 µl) |
| Preparation | Lyophilised purified protein |
| Recommended Dilution | Protein standard in 1D and 2D SDS gelelectrophoresis. Immunoassays. Immunization. Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS). |
| Protein Description | Recombinant human keratin K20 (formerly also designated cytokeratin 20). |
| Note | Isoelectric Point pI 5.66 |
| Storage | Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody at -20°C. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Reference Data | |
| RefSeq | NP_061883 |
| Locus ID | 54474 |
| UniProt ID | P35900 |
| Cytogenetics | 17q21.2 |
| Synonyms | Cytokeratin 20, Keratin-20, KRT20, CK20, K20, Protein IT |
| Summary | The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. provided by RefSeq, Jul 2008 |
| Protein Categories | Intracellular Proteins |
| Protein Families | 10 mM Tris-HCl, 2 mM dithiothreitol, both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, e.g. Dulbecco's PBS)., pH 7.4). For immunization purposes, Protein standard in 1D and 2D SDS gelelectrophoresis. Immunoassays. Immunization. Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml, the solution can be further dialyzed against PBS (phosphate buffered saline |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Citations
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