LRPAP1 / A2MRAP (His-tagged, myc-tagged) Rat Protein

CAT#: AR09364PU-L

LRPAP1 / A2MRAP (His-tagged, myc-tagged) rat protein, 0.5 mg

Specifications

Product Data

• Species: Rat
• Expression Host: E. coli
• Tag: His-tagged, myc-tagged
• Predicted MW: 40 kDa
• Concentration: lot specific
• Purity: >95% determined by SDS gelelectrophoresis
• Presentation: Purified
• Buffer: Presentation State: Purified
  State: Lyophilised purified protein
  Buffer System: TBS, pH 7.5, 0.1% BSA, 0.09% NaN3
• Reconstitution: Restore with 100 µl (100µg), 250 µl (250µg) or 500 µl (500µg) distilled water
• Preparation: Lyophilised purified protein
• Applications: Protein standard in 1D and 2D SDS gelelectrophoresis.
  Immunoblotting.
  Receptor-binding studies.
• Protein Description: Rat his:RAP:c-myc fusion protein
• Note: Ligand binding to RAP is Ca2+ dependent and e.g. lipid receptors can be released from RAP by a buffer containing 10 mM EDTA (cf. Bajari et al. 2005, see ref. below). Furthermore, buffers containing phosphate should be avoided (it would form precipitates with Ca2+).
• Storage: Prior to reconstitution store at 2-8°C.
  Following reconstitution store the antibody undiluted at -20°C for one month
  or (in aliquots) at -70°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_001162584
• Locus ID: 116565
• UniProt ID: Q99068
• Cytogenetics: 14q21
• Summary: Molecular chaperone for LDL receptor-related proteins that may regulate their ligand binding activity along the secretory pathway.[UniProtKB/Swiss-Prot Function]