Cytokeratin 14 Human Protein

CAT#: AR09363PU-S

Cytokeratin 14 human recombinant protein, 0.1 mg

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Concentration: lot specific
• Purity: >95% determined by SDS gelelectrophoresis
• Presentation: Purified
• Buffer: Presentation State: Purified
  State: Lyophilized purified protein
• Reconstitution: AR09363PU-N: Restore with 175 µl distilled water (final volume 250 µl).
  AR09363PU-S: Restore with 70 µl distilled water (final volume 100 µl).
  Final solution: 30mM Tris/HCI pH 8, 9.5M Urea, 2mM DTT, 2mM EDTA, 10mM Methylammonium Chloride.
• Preparation: Lyophilized purified protein
• Applications: Protein standard in 1D and 2D SDS gelelectrophoresis.
  Immunoassays.
  Immunization.
  Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4).
  For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).
• Protein Description: Recombinant human keratin K14 (formerly also designated cytokeratin 14).
  Molecular weight:
  51,662 (+ methionin).
  51,530 (- methionin; calculated from sequence).
  50,000 (determined by SDS gelelectrophoresis).
• Note: Isoelectric Point: pI 5.3 (4.92 calculated from sequence)
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_000517
• Locus ID: 3861
• UniProt ID: P02533
• Cytogenetics: 17q21.2
• Synonyms: CK14; EBS3; EBS4; K14; NFJ
• Summary: This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. [provided by RefSeq, Jul 2008]