Desmin Human Protein

CAT#: AR09361PU-N

Desmin human recombinant protein, 0.25 mg

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Predicted MW: 53,539
• Concentration: 1.0 mg/ml (after reconstitution)
• Purity: >95% determined by SDS gelelectrophoresis
• Presentation: Purified
• Buffer: Presentation State: Purified
  State: Lyophilised purified protein
  Buffer System: 30 mM Tris/HCI pH 8, 9.5 M urea, 2 mM DTT, 2 mM EDTA, 10 mM methylammonium chloride
• Reconstitution: Reconstitute with 175 µl distilled water (final volume 250 µl)
• Preparation: Lyophilised purified protein
• Applications: Protein standard in 1D and 2D SDS gelelectrophoresis.
  Immunoassays.
  Immunization.
  Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above), protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).
• Protein Description: Recombinant human desmin
• Note: Isoelectric Point pI 5.16 (calculated from sequence)
• Storage: Prior to reconstitution store at 2-8°C.
  Following reconstitution store the antibody at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_001918
• Locus ID: 1674
• UniProt ID: P17661, Q53SB5
• Cytogenetics: 2q35
• Synonyms: CDCD3; CSM1; CSM2; LGMD1D; LGMD1E; LGMD2R
• Summary: This gene encodes a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies. [provided by RefSeq, Jul 2008]
• Protein Families: Druggable Genome
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM)