Cyclophilin G / PPIG (1-175, His-Tag) Human Protein
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | E. coli |
| Expression cDNA Clone or AA Sequence | |
| Tag | His-Tag |
| Predicted MW | 21.6 kDa (195aa) confirmed by MALDI-TOF |
| Concentration | 1mg/ml (determined by Bradford assay) |
| Purity | > 95% by SDS-PAGE |
| Presentation State | Purified |
| Buffer | Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol |
| Bioactivity | Specific activity is > 600nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmol of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin. |
| Preparation | Liquid purified protein |
| Recommended Dilution | SDS-PAGE, Enzyme Activity |
| Protein Description | Recombinant human Cyclophilin G, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. |
| Storage | Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Reference Data | |
| RefSeq | AAH01555 |
| Locus ID | 9360 |
| UniProt ID | Q13427 |
| Cytogenetics | 2q31.1 |
| Synonyms | Peptidyl-prolyl cis-trans isomerase G, PPIase G, Rotamase G, Cyclophilin G, CASP10, PPIG, Peptidyl-prolyl cis-trans isomerase G,peptidylprolyl isomerase G (cyclophilin G), CARS-Cyp, SRCyp, SCAF10, SR-related CTD-associated factor 10 |
| Summary | PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding (PubMed:20676357). May be implicated in the folding, transport, and assembly of proteins. May play an important role in the regulation of pre-mRNA splicing.UniProtKB/Swiss-Prot Function |
| Protein Categories | Intracellular Proteins |
| Protein Families | pH 8.0, Protocol: Activity Assay 1. Prepare 170 ul assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-HCl |
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Resources
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