Cyclophilin G / PPIG (1-175, His-Tag) Human Protein

SKU
AR09314PU-N
Recombinant human Cyclophilin G/PPIG protein
  $410.00
3 Weeks*
Bulk/Customize
Specifications
Specifications
Product Data
Species Human
Expression Host E. coli
Expression cDNA Clone or AA Sequence
Protein Sequence
MGSSHHHHHH SSGLVPRGSH MGIKVQRPRC FFDIAINNQP AGRVVFELFS DVCPKTCENF RCLCTGEKGT GKSTQKPLHY KSCLFHRVVK DFMVQGGDFS EGNGRGGESI YGGFFEDESF AVKHNKEFLL SMANRGKDTN GSQFFITTKP TPHLDGHHVV FGQVISGQEV VREIENQKTD AASKPFAEVR ILSCG
Tag His-Tag
Predicted MW 21.6 kDa (195aa) confirmed by MALDI-TOF
Concentration 1mg/ml (determined by Bradford assay)
Purity > 95% by SDS-PAGE
Presentation State Purified
Buffer Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol
Bioactivity Specific activity is > 600nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmol of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.
Preparation Liquid purified protein
Recommended Dilution SDS-PAGE, Enzyme Activity
Protein Description Recombinant human Cyclophilin G, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Storage Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Reference Data
RefSeq AAH01555
Locus ID 9360
UniProt ID Q13427
Cytogenetics 2q31.1
Synonyms Peptidyl-prolyl cis-trans isomerase G, PPIase G, Rotamase G, Cyclophilin G, CASP10, PPIG, Peptidyl-prolyl cis-trans isomerase G,peptidylprolyl isomerase G (cyclophilin G), CARS-Cyp, SRCyp, SCAF10, SR-related CTD-associated factor 10
Summary PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding (PubMed:20676357). May be implicated in the folding, transport, and assembly of proteins. May play an important role in the regulation of pre-mRNA splicing.UniProtKB/Swiss-Prot Function
Protein Categories Intracellular Proteins
Protein Families pH 8.0, Protocol: Activity Assay
1. Prepare 170 ul assay buffer into a suitable container and pre-chill on ice before use:
The final concentrations are 200 mM Tris-HCl
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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