Galectin-4 (1-323, His-tag) Human Protein

CAT#: AR09292PU-N

Galectin-4 (1-323, His-tag) human recombinant protein, 0.1 mg

Product Images

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MGSSHHHHHH SSGLVPRGSH MAYVPAPGYQ PTYNPTLPYY QPIPGGLNVG MSVYIQGVAS EHMKRFFVNF VVGQDPGSDV AFHFNPRFDG WDKVVFNTLQ GGKWGSEERK RSMPFKKGAA FELVFIVLAE HYKVVVNGNP FYEYGHRLPL QMVTHLQVDG DLQLQSINFI GGQPLRPQGP PMMPPYPGPG HCHQQLNSLP TMEGPPTFNP PVPYFGRLQG GLTARRTIII KGYVPPTGKS FAINFKVGSS GDIALHINPR MGNGTVVRNS LLNGSWGSEE KKITHNPFGP GQFFDLSIRC GLDRFKVYAN GQHLFDFAHR LSAFQRVDTL EIQGDVTLSY VQI
• Tag: His-tag
• Predicted MW: 38.1 kDa
• Concentration: lot specific
• Purity: >95% by SDS – PAGE
• Presentation: Purified
• Buffer: Presentation State: Purified
  State: Liquid purified protein
  Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1 mM DTT
• Bioactivity: Specific: The ED50 for this effect is is 10 – 20 ug/ml.
  Measured by i its ability to agglutinate human red blood cells.
  Biological Assay :
  1. Mix equal volumes of human blood and Alsever’s solution (pH 7.0). (Alsever’s solution: NaCl 0.42 g, Sodium citric acid 0.8g, Citric acid 0.055 g, D - glucose 2.05g in D W 100 ml)
  2. Centrifuge at 15,000 rpm for 10 minutes and wash four times with PBS.
  3. Dilute packed cells in a 0.5 mg/ml trypsin - EDTA solution to give 4% re d cell suspension.
  4. Incubate for 1h r at 37°C and wash four times with PBS.
  5 . Dilute packed cells in PBS to give 4% red cell suspension.
  6 . Load 50ul of 0.5% BSA - in - 0.15M - NaCl solution and 25ul of 4% - Red - Cell - in - PBS in u shaped wells.
• Preparation: Liquid purified protein
• Protein Description: Recombinant Galectin-4 protein, fused to His-tag, was expressed in E.coli and purified by using conventional chromatography techniques.
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_006140
• Locus ID: 3960
• UniProt ID: P56470, Q6FHZ4
• Cytogenetics: 19q13.2
• Synonyms: GAL4; L36LBP
• Summary: The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The expression of this gene is restricted to small intestine, colon, and rectum, and it is underexpressed in colorectal cancer. [provided by RefSeq, Jul 2008]