PEBP1 / RKIP (1-187) Human Protein

SKU
AR09265PU-N
Recombinant human RKIP/PEBP1 protein
  $355.00
3 Weeks*
Bulk/Customize
Specifications
Specifications
Product Data
Species Human
Expression Host E. coli
Expression cDNA Clone or AA Sequence
Protein Sequence
MPVDLSKWSG PLSLQEVDEQ PQHPLHVTYA GAAVDELGKV LTPTQVKNRP TSISWDGLDS GKLYTLVLTD PDAPSRKDPK YREWHHFLVV NMKGNDISSG TVLSDYVGSG PPKGTGLHRY VWLVYEQDRP LKCDEPILSN RSGDHRGKFK VASFRKKYEL RAPVAGTCYQ AEWDDYVPKL YEQLSGK
Predicted MW 21 kDa (187aa) confirmed by MALDI-TOF
Concentration 1mg/ml (determined by Bradford assay)
Purity > 95% by SDS-PAGE
Presentation State Purified
Buffer Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol
Endotoxin < 1 EU per 1ug of protein (determined by LAL method)
Preparation Liquid purified protein
Recommended Dilution SDS-PAGE
Protein Description Recombinant human PEBP1 was expressed in E.coli and purified by using conventional chromatography techniques.
Storage Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Reference Data
RefSeq NP_002558
Locus ID 5037
UniProt ID P30086
Cytogenetics 12q24.23
Synonyms Phosphatidylethanolamine binding protein 1, PBP, Prostatic binding protein, RKIP, HCNP, HCNPpp, PEBP, Raf kinase inhibitory protein, Hippocampal cholinergic neurostimulating peptide
Summary This gene encodes a member of the phosphatidylethanolamine-binding family of proteins and has been shown to modulate multiple signaling pathways, including the MAP kinase (MAPK), NF-kappa B, and glycogen synthase kinase-3 (GSK-3) signaling pathways. The encoded protein can be further processed to form a smaller cleavage product, hippocampal cholinergic neurostimulating peptide (HCNP), which may be involved in neural development. This gene has been implicated in numerous human cancers and may act as a metastasis suppressor gene. Multiple pseudogenes of this gene have been identified in the genome. provided by RefSeq, Jul 2015
Protein Categories CAR-T Targets, Intracellular Proteins
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Documents
Resources

file-alt Citations

Phosphatidylethanolamine Binding Protein 1 (PEBP1) in Alzheimer's Disease: ELISA Development and Clinical Validation
Wojdała, Chiasserini, Bellomo et al
J Alzheimers Dis (2022) 88 (4), 1459-1468 DOI: 10.3233/JAD-220323
AI Summary: OriGene’s human recombinant PEBP1 protein (AR09265PU-N) was used as a standard control in Western blot experiments to validate antibody specificity during the development of the novel ELISA for PEBP1 detection in this study.
Snippet: trifuged for 10 min at 16,000× g. Supernatant was collected and the total protein concentration was measured by the Bradford assay. Finally, 100 ng human recombinant protein PEBP1 (AR09265PU-N, OriGene, USA) was prepared by diluting it in 20 mM Tris-HCl (pH 8), 150 mM NaCl. For each anti-PEBP1 antibody (sc-376925/372100/HPA063904) the WB experiment was performed separately. A 12% polyac
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