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Profilin-1 / PFN1 (1-140) Human Protein

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SKU
AR09259PU-L
Recombinant human Profilin 1/PFN1 protein
Size:
500 ug
  $830.00
3 Weeks*
Bulk/Customize

Other products for "PFN1 or NM_005022"

Specifications
Specifications
Product Data
Species Human
Expression Host E. coli
Expression cDNA Clone or AA Sequence
Protein Sequence
MAGWNAYIDN LMADGTCQDA AIVGYKDSPS VWAAVPGKTF VNITPAEVGV LVGKDRSSFY VNGLTLGGQK CSVIRDSLLQ DGEFSMDLRT KSTGGAPTFN VTVTKTDKTL VLLMGKEGVH GGLINKKCYE MASHLRRSQY
Predicted MW 15 kDa (140aa) confirmed by MALDI-TOF
Concentration 1mg/ml (determined by Bradford assay)
Purity > 95% by SDS-PAGE
Presentation State Purified
Buffer Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Endotoxin < 1 EU per 1ug of protein (determined by LAL method)
Preparation Liquid purified protein
Recommended Dilution SDS-PAGE
Protein Description Recombinant Profilin1 protein was expressed in E.coli and purified by using conventional chromatography techniques.
Storage Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Stability Shelf life: one year from despatch
Shipping Blue Ice
Reference Data
RefSeq NP_005013
Locus ID 5216
UniProt ID P07737
Cytogenetics 17p13.2
Synonyms Profilin-1, Profilin I, Profilin, Pfn1, Actin binding protein
Summary This gene encodes a member of the profilin family of small actin-binding proteins. The encoded protein plays an important role in actin dynamics by regulating actin polymerization in response to extracellular signals. Deletion of this gene is associated with Miller-Dieker syndrome, and the encoded protein may also play a role in Huntington disease. Multiple pseudogenes of this gene are located on chromosome 1. provided by RefSeq, Jul 2012
Protein Categories Intracellular Proteins, Nervous system: Neurodegenerative diseases
Protein Families Druggable Genome, Stem cell - Pluripotency
Protein Pathways Regulation of actin cytoskeleton
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Documents
Resources

file-alt Citations

The effect of piperlongumine on endothelial and lung adenocarcinoma cells with regulated expression of profilin-1
Gagat, Hałas-Wiśniewska, Zielińska et al
Onco Targets Ther (2018) 11, 8275-8292 DOI: 10.2147/OTT.S183191
AI Summary: OriGene products, specifically the expression plasmid with cloned cDNA of PFN1 and the empty control plasmid vector, were used to upregulate profilin-1 expression and serve as controls in endothelial and lung adenocarcinoma cell experiments in this study.
Snippet: In order to upregulate (EA.hy926) and downregulate (A549) the level of PFN1 expression, the cells were transfected using expression plasmid with cloned cDNA of PFN1 (OriGene, Rockville, MD, USA) and siRNA against PFN1 (Qiagen, Hilden, Germany), respectively;In order to upregulate (EA.hy926) and downregulate (A549) the level of PFN1 expression, the cells were transfected using expression plasmid with cloned cDNA of PFN1 (OriGene, Rockville, MD, USA) and siRNA against PFN1 (Qiagen, Hilden, Germany), respectively. For determining the unspecific effect of the overexpression and loss of PFN1, the cells were transfected with empty control plasmid vector (OriGene)
FigureFigureFigure
Phosphoglycerate mutase 1 promotes cancer cell migration independent of its metabolic activity
Zhang, Jin, Sun et al
Oncogene (2016)
AI Summary: In this study, OriGene’s ACTA2 and Profilin-1 recombinant proteins were used in GST pull-down assays to investigate and confirm the direct interaction between PGAM1 and ACTA2, elucidating PGAM1's non-metabolic role in promoting cancer cell migration.
Snippet: GST-pull down assay. In total, 10 μg purified GST fused PGAM1 was incubated with 200 ng ACTA2 (Origene, Rockville, MD, USA) or with the same amount of Profilin-1 (Origene) protein in 200 μl binding buffer (20 mm Tris-HCl pH 8.0, 150 mm NaCl, 1% Nonidet P40 ... Cite SaveSaving
Fluorescence Resonance Energy Transfer (FRET)-based Detection of Profilin-VASP Interaction
Gau, Ding, Baty et al
Cell Mol Bioeng (2011) 4 (1), 1-8
Cited by 4
AI Summary: OriGene's pRFP-C-RS vector was used to construct Pfn1-shRNA for targeted gene silencing in the study investigating Profilin-VASP interactions using FRET.
Snippet: sites. Pfn1-shRNA was constructed by ligating a commercially available vector pRFP-C-RS (Origene Technologies Inc., Rockville, MD) with a custom-designed 21-mer hairpin sequence targeting Pfn1 at BamH1 and Hind3 sites.
Showing 1-3 of 3 papers.
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