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GSTP1 / GST3 (1-210, His-Tag) Human Protein

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SKU
AR09222PU-L
Recombinant human Glutathione S-Transferase pi 1/GSTP1 protein
Size:
500 ug
  $1,480.00
3 Weeks*
Bulk/Customize

Other products for "GSTP1 or NM_000852"

Specifications
Specifications
Product Data
Species Human
Expression Host E. coli
Expression cDNA Clone or AA Sequence
Protein Sequence
MPPY TVVYFPVRGR CAALRMLLAD QGQSWKEEVV TVETWQEGSL KASCLYGQLP KFQDGDLTLY QSNTILRHLG RTLGLYGKDQ QEAALVDMVN DGVEDLRCKY ISLIYTNYEA GKDDYVKALP GQLKPFETLL SQNQGGKTFI VGDQISFADY NLLDLLLIHE VLAPGCLDAF PLLSAYVGRL SARPKLKAFL ASPEYVNLPI NGNGKQ
Tag His-Tag
Predicted MW 27.4 kDa (246aa) confirmed by MALDI-TOF
Concentration 0.5mg/ml (determined by Bradford assay)
Purity > 95% by SDS-PAGE
Presentation State Purified
Buffer Liquid in. 20mM Tris-HCl buffer (pH 7.0) containing 30% glycerol, 1mM EDTA, 0.1mM PMSF.
Bioactivity Specific activity is > 80unit/mg, and is defined as the amount of enzyme that conjugate 1.0 umole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.
Endotoxin < 1 EU per 1ug of protein (determined by LAL method)
Preparation Liquid purified protein
Recommended Dilution SDS-PAGE, Enzyme Activity
Protein Description Recombinant GSTP1 protein, fused to His-tag, was expressed in E.coli and purified by using conventional chromatography techniques.
Storage Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Reference Data
RefSeq NP_000843
Locus ID 2950
UniProt ID P09211
Cytogenetics 11q13.2
Synonyms SERPINF1, Serpin peptidase inhibitor clade F member 1, Proliferation inducing protein 35, Pigment epithelium-derived factor EPC 1, Pigment epithelium-derived factor, PIG35, PI, GSTP1, GST3, Glutathione S-Transferase Pi 1, Glutathione S-transferase P Fatty Acid Ethyl Ester Synthase III, Glutathione S-transferase P, Glutathione S Transferase Pi, FAEES3, EPC1, DFN7, APF0619
Summary Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This GST family member is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases. provided by RefSeq, Jul 2008
Protein Categories Intracellular Proteins
Protein Families Druggable Genome
Protein Pathways Drug metabolism - cytochrome P450, Glutathione metabolism, Metabolism of xenobiotics by cytochrome P450, Pathways in cancer, Prostate cancer
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Documents
Resources

file-alt Citations

Glutathione S-Transferase P1 Protects Against Amodiaquine Quinoneimines-Induced Cytotoxicity but Does Not Prevent Activation of Endoplasmic Reticulum Stress in HepG2 Cells
Zhang, den Braver-Sewradj, den Braver et al
Front Pharmacol (2018) 9, 388 DOI: 10.3389/fphar.2018.00388
AI Summary: OriGene’s hGSTP1 expression plasmid (SC119655) and empty pCMV6-XL5 vector were used to transiently transfect HepG2 cells, enabling the study of GSTP1’s protective effects against amodiaquine quinoneimine-induced cytotoxicity in this research.
Snippet: After plating on collagen-coated plates for 24 h, HepG2 cells were transiently transfected with 0.1 μg/1 × 104 cells hGSTP1 expression plasmid (SC119655, Origene, Rockville, MD, United States) or accompanying empty pCMV6-XL5 vector (pCMV6-XL5) using the GenJet In Vitro Transfection Reagent for HepG2 cells (SignaGen, Rockville, MD, United States) according to the manufacturer’s instructions. …
FigureFigureFigure
Human NAD(P)H:quinone Oxidoreductase 1 (NQO1)-Mediated Inactivation of Reactive Quinoneimine Metabolites of Diclofenac and Mefenamic Acid
Vredenburg, Elias, Venkataraman et al
Chem. Res. Toxicol. (2014)
Cited by 29
AI Summary: OriGene's pCMV6-XL5 plasmid carrying the human NQO1 gene (catalog number SC119599) was used to clone, express, and purify human NQO1, enabling the study of its role in detoxifying reactive drug metabolites.
Snippet: Page 9. 9 2.2 Cloning, expression and purification of human NQO1 Plasmid pCMV6-XL5, carrying the human NQO1 gene, was obtained from OriGene Technologies (SC119599;Rockville, MD). The NQO1 gene was excised from its original vector ... Cite SaveSaving
Showing 1-2 of 2 papers.
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