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Peroxiredoxin-3 / PRDX3 (63-256) Human Protein

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SKU
AR09202PU-L
Recombinant human Peroxiredoxin 3/PRDX3 protein
Size:
500 ug
  $1,480.00
3 Weeks*
Bulk/Customize

Other products for "PRDX3 or NM_001302272"

Specifications
Specifications
Product Data
Species Human
Expression Host E. coli
Expression cDNA Clone or AA Sequence
Protein Sequence
MPAVTQHAPY FKGTAVVNGE FKDLSLDDFK GKYLVLFFYP LDFTFVCPTE IVAFSDKANE FHDVNCEVVA VSVDSHFSHL AWINTPRKNG GLGHMNIALL SDLTKQISRD YGVLLEGSGL ALRGLFIIDP NGVIKHLSVN DLPVGRSVEE TLRLVKAFQY VETHGEVCPA NWTPDSPTIK PSPAASKEYF QKVNQ
Predicted MW 21.5 kDa (195aa) confirmed by MALDI-TOF
Concentration 1mg/ml (determined by Bradford assay)
Purity > 95% by SDS-PAGE
Presentation State Purified
Buffer Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Bioactivity Specific activity is >2,000pmol/min/ug. Enzymatic activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25C for 1minute.
Preparation Liquid purified protein
Recommended Dilution SDS-PAGE, Enzyme Activity
Protein Description Recombinant human Peroxiredoxin 3 protein was expressed in E.coli and purified by using conventional chromatography techniques.
Storage Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Reference Data
RefSeq NP_006784
Locus ID 10935
UniProt ID P30048
Cytogenetics 10q26.11
Synonyms Thioredoxin-dependent peroxide reductase mitochondrial, Thioredoxin-dependent peroxide reductase mitochondrial, PRDX3, PRX III, Antioxidant protein 1 (AOP-1), Peroxiredoxin 3, Thioredoxin-dependent peroxide reductase, mitochondrial Antioxidant Protein 1, AOP1, MER5, PRX3, SP22, Peroxiredoxin-3
Summary This gene encodes a mitochondrial protein with antioxidant function. The protein is similar to the C22 subunit of Salmonella typhimurium alkylhydroperoxide reductase, and it can rescue bacterial resistance to alkylhydroperoxide in E. coli that lack the C22 subunit. The human and mouse genes are highly conserved, and they map to the regions syntenic between mouse and human chromosomes. Sequence comparisons with recently cloned mammalian homologs suggest that these genes consist of a family that is responsible for the regulation of cellular proliferation, differentiation and antioxidant functions. This family member can protect cells from oxidative stress, and it can promote cell survival in prostate cancer. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1, 3, 13 and 22. provided by RefSeq, Oct 2014
Protein Categories Intracellular Proteins, Nervous system: Neurodegenerative diseases
Protein Families 0.03X PBS, 0.5% glycerol.
2. Add 5 ul of recombinant PRDX3 solution with various concentrations (0.25ug, 2.5M KSCN)
7. Record the increase in A475nm., Protocol: Activity Assay:
1. Prepare a 50ul reaction mix into a suitable container :
The final concentrations are 1mM DTT
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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