Staphylococcal protein A (37-469) S.aureus Protein
Specifications
| Product Data | |
| Species | S.aureus |
|---|---|
| Expression Host | E. coli |
| Expression cDNA Clone or AA Sequence | |
| Predicted MW | 48.1 kDa (434aa) confirmed by MALDI-TOF |
| Concentration | 1mg/ml (determined by BCA assay) |
| Purity | > 90% by SDS-PAGE |
| Presentation State | Purified |
| Buffer | Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol |
| Endotoxin | < 1 EU per 1ug of protein (determined by LAL method) |
| Preparation | Liquid purified protein |
| Recommended Dilution | SDS-PAGE |
| Protein Description | Recombinant Staphylococcus aureus Protein A was expressed in E.coli and purified by using conventional chromatography. |
| Storage | Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Reference Data | |
| RefSeq | ACD80064 |
| UniProt ID | B3GGB2 |
| Synonyms | Immunoglobulin G binding protein A, Immunoglobulin G binding protein A, Immunoglobulin G binding protein A Staphylococcal protein A, SPA |
| Summary | Protein A is a 40-60 kDa surface protein originally found in the cell wall of the bacteria Staphylococcus aureus. It binds with high affinity to human IgG1 and IgG2 as well as mouse IgG2a and IgG2b. Protein A binds with moderate affinity to human IgM, IgA and IgE as well as to mouse IgG3 and IgG1. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology. Recombinant Staphylococcus aureus Protein A was expressed in E. coli and purified by using conventional chromatography. |
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