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ALDH2 (18-517) Human Protein

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SKU
AR09142PU-N
Recombinant human Aldehyde dehydrogenase 2/ALDH2 protein
Size:
100 ug
  $355.00
3 Weeks*
Bulk/Customize

Other products for "ALDH2 or NM_000690"

Specifications
Specifications
Product Data
Species Human
Expression Host E. coli
Expression cDNA Clone or AA Sequence
Protein Sequence
MSAAATQAVP APNQQPEVFC NQIFINNEWH DAVSRKTFPT VNPSTGEVIC QVAEGDKEDV DKAVKAARAA FQLGSPWRRM DASHRGRLLN RLADLIERDR TYLAALETLD NGKPYVISYL VDLDMVLKCL RYYAGWADKY HGKTIPIDGD FFSYTRHEPV GVCGQIIPWN FPLLMQAWKL GPALATGNVV VMKVAEQTPL TALYVANLIK EAGFPPGVVN IVPGFGPTAG AAIASHEDVD KVAFTGSTEI GRVIQVAAGS SNLKRVTLEL GGKSPNIIMS DADMDWAVEQ AHFALFFNQG QCCCAGSRTF VQEDIYDEFV ERSVARAKSR VVGNPFDSKT EQGPQVDETQ FKKILGYINT GKQEGAKLLC GGGIAADRGY FIQPTVFGDV QDGMTIAKEE IFGPVMQILK FKTIEEVVGR ANNSTYGLAA AVFTKDLDKA NYLSQALQAG TVWVNCYDVF GAQSPFGGYK MSGSGRELGE YGLQAYTEVK TVTVKVPQKN S
Predicted MW 54.5 kDa (501aa) confirmed by MALDI-TOF
Concentration 1mg/ml (determined by absorbance at 280nm)
Purity > 90% by SDS-PAGE
Presentation State Purified
Buffer Liquid in. 20mM Tris-HCl buffer (pH 7.5) containing 1mM DTT, 1mM EDTA, 10% glycerol
Bioactivity Specific activity is > 250pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0 pmole Acetaldehyde by NAD per minute at pH 8.0 at 25℃.
Endotoxin < 1 EU per 1ug of protein (determined by LAL method)
Preparation Liquid purified protein
Recommended Dilution SDS-PAGE, Enzyme Activity
Protein Description Recombinant Human ALDH2 protein was expressed in E.coli and purified by using conventional chromatography techniques.
Storage Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Reference Data
RefSeq NP_000681.2
Locus ID 217
UniProt ID P05091
Cytogenetics 12q24.12
Synonyms Aldehyde dehydrogenase 2 family member, Aldehyde dehydrogenase mitochondrial, ALDH class 2, ALDH-E2, ALDHI, ALDM
Summary This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of East Asians have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among East Asians than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. Alternative splicing results in multiple transcript variants encoding distinct isoforms.provided by RefSeq, Nov 2016
Protein Categories Intracellular Proteins
Protein Families 10 mM 2-mercaptoethanol, 100 mM potassium chloride, 2 mM acetaldehyde
2. Equilibrate to 25°C and monitor the A340nm until value is constant using a spectrophotometer.
3. Add 20 ul of recombinant ALDH2 protein 400ug/ml in assay buffer.
4. Record the increase in A340nm for 5 minutes., 2 mM beta-NAD, Protocol: Activity Assay:
1. Prepare a 180 µl assay buffer into a suitable container: The concentrations are 100 mM Tris-HCl (pH 8.0)
Protein Pathways Arginine and proline metabolism, Ascorbate and aldarate metabolism, beta-Alanine metabolism, Butanoate metabolism, Fatty acid metabolism, Glycerolipid metabolism, Glycolysis / Gluconeogenesis, Histidine metabolism, leucine and isoleucine degradation, Limonene and pinene degradation, Lysine degradation, Metabolic pathways, Propanoate metabolism, Pyruvate metabolism, Tryptophan metabolism, Valine
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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