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NEDD8 (1-76, His-Tag) Human Protein

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SKU
AR09126PU-N
Recombinant human NEDD8 protein
Size:
100 ug
  $455.00
3 Weeks*
Bulk/Customize

Other products for "NEDD8 or NM_006156"

Specifications
Specifications
Product Data
Species Human
Expression Host E. coli
Expression cDNA Clone or AA Sequence
Protein Sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMLI KVKTLTGKEI EIDIEPTDKV ERIKERVEEK EGIPPQQQRL IYSGKQMNDE KTAADYKILG GSVLHLVLAL RGG
Tag His-Tag
Predicted MW 12.8 kDa (113aa) confirmed by MALDI-TOF
Concentration 0.5mg/ml (determined by Bradford assay)
Purity > 95% by SDS-PAGE
Presentation State Purified
Buffer Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.5mM DTT, 50mM NaCl, 10% glycerol
Endotoxin < 1 EU per 1ug of protein (determined by LAL method)
Preparation Liquid purified protein
Recommended Dilution SDS-PAGE
Protein Description Recombinant human NEDD8 protein, fused to His-tag, was expressed in E.coli and purified by using conventional chromatography techniques, after refolding of the isolated inclusion body in renaturation buffer.
Storage Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Reference Data
RefSeq NP_006147
Locus ID 4738
UniProt ID Q15843
Cytogenetics 14q12
Synonyms Neural precursor cell expressed developmentally down-regulated 8, ubiquitin-like protein Nedd8, Neddylin, Neural precursor cell expressed, developmentally down-regulated 8 MGC104393, MGC125896, MGC125897, NED8, 0NEDD 8, Neddylin, Neural precursor cell expressed developmentally down regulated 8, Neural precursor cell expressed developmentally down regulated gene 8, Rub1, ubiquitin like protein Nedd 8, ubiquitin like protein Nedd8.
Summary Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.UniProtKB/Swiss-Prot Function
Protein Categories Intracellular Proteins
Protein Families Druggable Genome
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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