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Fumarase (44-510) Human Protein

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SKU
AR09123PU-L
Recombinant human Fumarase/FH protein
Size:
500 ug
  $830.00
3 Weeks*
Bulk/Customize

Other products for "FH or NM_000143"

Specifications
Specifications
Product Data
Species Human
Expression Host E. coli
Expression cDNA Clone or AA Sequence
Protein Sequence
MASQNSFRIE YDTFGELKVP NDKYYGAQTV RSTMNFKIGG VTERMPTPVI KAFGILKRAA AEVNQDYGLD PKIANAIMKA ADEVAEGKLN DHFPLVVWQT GSGTQTNMNV NEVISNRAIE MLGGELGSKI PVHPNDHVNK SQSSNDTFPT AMHIAAAIEV HEVLLPGLQK LHDALDAKSK EFAQIIKIGR THTQDAVPLT LGQEFSGYVQ QVKYAMTRIK AAMPRIYELA AGGTAVGTGL NTRIGFAEKV AAKVAALTGL PFVTAPNKFE ALAAHDALVE LSGAMNTTAC SLMKIANDIR FLGSGPRSGL GELILPENEP GSSIMPGKVN PTQCEAMTMV AAQVMGNHVA VTVGGSNGHF ELNVFKPMMI KNVLHSARLL GDASVSFTEN CVVGIQANTE RINKLMNESL MLVTALNPHI GYDKAAKIAK TAHKNGSTLK ETAIELGYLT AEQFDEWVKP KDMLGPK
Predicted MW 50.2 kDa (467aa) confirmed by MALDI-TOF
Concentration 1mg/ml (determined by Bradford assay)
Purity > 95% by SDS-PAGE
Presentation State Purified
Buffer Liquid in. 20mM Tris-HCl buffer (pH 8.0)
Bioactivity Specific activity is > 25 unit/mg, and is defined as the amount of enzyme that cleaves 1umole of L-Malate to Fumarate per minute at pH 7.5 at 37C.
Endotoxin < 1 EU per 1ug of protein (determined by LAL method)
Preparation Liquid purified protein
Recommended Dilution SDS-PAGE, Enzyme Activity
Protein Description Recombinant Fumarase was expressed in E.coli and was purified by conventional chromatography techniques.
Storage Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Reference Data
RefSeq NP_000134
Locus ID 2271
UniProt ID P07954
Cytogenetics 1q43
Synonyms Fumarate hydratase, FH, HLRCC, LRCC, MCL, MCuL1, Fumarate hydratase Fumarase, Fumarate hydratase mitochondrial, MCuL 1, Multiple hereditary cutaneous leiomyomata.
Summary The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. provided by RefSeq, Jul 2008
Protein Categories Cancer: Bladder, Cancer: Endocrine, Endocrine and metabolic diseases, Intracellular Proteins
Protein Families 1ug, 2ug) in assay buffer.
3. Record the increase in A260 nm for 10 minutes
- L-Malate (Sigma-Aldrich. Cat. No, 3635)
- plate reader (PerkinElmer, 50 mM L-malic acid).
2. Add 50 ul of recombinant Fumarase protein with various concentrations (0.5 ug, Cat. No, M-1000)
- 96 well UV plate (Costar, Protocol: Activity Assay
1. Prepare 1.45 ml assay buffer (Assay buffer: 100 mM potassium phosphate, VICTORX3)
Protein Pathways Citrate cycle (TCA cycle), Metabolic pathways, Pathways in cancer, Renal cell carcinoma
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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