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PPIL1 (1-166, His-Tag) Human Protein

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SKU
AR09117PU-N
Recombinant human Cyclophilin-like 1/PPIL1 protein
Size:
100 ug
  $355.00
3 Weeks*
Bulk/Customize

Other products for "PPIL1 or NM_016059"

Specifications
Specifications
Product Data
Species Human
Expression Host E. coli
Expression cDNA Clone or AA Sequence
Protein Sequence
MAAIPPDSWQ PPNVYLETSM GIIVLELYWK HAPKTCKNFA ELARRGYYNG TKFHRIIKDF MIQGGDPTGT GRGGASIYGK QFEDELHPDL KFTGAGILAM ANAGPDTNGS QFFVTLAPTQ WLDGKHTIFG RVCQGIGMVN RVGMVETNSQ DRPVDDVKII KAYPSG
Tag His-Tag
Predicted MW 19.3 kDa (174aa) confirmed by MALDI-TOF
Concentration 1mg/ml (determined by Bradford assay)
Purity > 95% by SDS-PAGE
Presentation State Purified
Buffer Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol
Bioactivity Specific activity is > 700nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.
Preparation Liquid purified protein
Recommended Dilution SDS-PAGE, Enzyme Activity
Protein Description Recombinant PPIL1, fused to His-tag at C-terminus, was expressed in E.coli and purified by conventional chromatography techniques.
Storage Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Reference Data
RefSeq NP_057143
Locus ID 51645
UniProt ID Q9Y3C6
Cytogenetics 6p21.2
Synonyms Peptidyl-prolyl cis-trans isomerase-like 1, Peptidylprolyl isomerase like 1, Cyclophilin like 1, CYPL1, Rotamase PPIL1, PPIase
Summary This gene is a member of the cyclophilin family of peptidylprolyl isomerases (PPIases). The cyclophilins are a highly conserved, ubiquitous family, members of which play an important role in protein folding, immunosuppression by cyclosporin A, and infection of HIV-1 virions. Based on similarity to other PPIases, this protein could accelerate the folding of proteins and might catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. provided by RefSeq, Jul 2008
Protein Categories Intracellular Proteins
Protein Families pH 8.0, Protocol: Activity Assay
1. Prepare 170 ul assay buffer into a suitable container and pre-chill on ice before use:
The final concentrations are 200 mM Tris-HCl
Protein Pathways Spliceosome
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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