Thioredoxin-2 / TRX2 (60-166) Human Protein
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | E. coli |
| Expression cDNA Clone or AA Sequence | |
| Predicted MW | 11 kDa (108aa) confirmed by MALDI-TOF |
| Concentration | 1mg/ml (determined by Bradford assay) |
| Purity | > 95% by SDS-PAGE |
| Presentation State | Purified |
| Buffer | Liquid in. Phosphate-Buffered Saline (pH 7.4) |
| Bioactivity | Specific activity is >40 A650/cm/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin. |
| Preparation | Liquid purified protein |
| Recommended Dilution | Enzyme Activity,SDS-PAGE |
| Protein Description | Recombinant Thioredoxin2 was expressed in E.coli and purified by using conventional chromatography techniques. |
| Storage | Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Reference Data | |
| RefSeq | NP_036605 |
| Locus ID | 25828 |
| UniProt ID | Q99757 |
| Cytogenetics | 22q12.3 |
| Synonyms | MTRX, TRX2, MT-TRX, Thioredoxin2, mitochondrial thioredoxin, thioredoxin 2 precursor, Thioredoxin mitochondrial, TRX 2, TXN 2, TXN2, Thioredoxin-2 |
| Summary | This nuclear gene encodes a mitochondrial member of the thioredoxin family, a group of small multifunctional redox-active proteins. The encoded protein may play important roles in the regulation of the mitochondrial membrane potential and in protection against oxidant-induced apoptosis. provided by RefSeq, Jul 2008 |
| Protein Categories | Intracellular Proteins |
| Protein Families | 0.1% (w/v) insulin, 1mM DTT, 2mM EDTA. 2. Add 4 ug of recombinant thioredoxin2 into reaction cocktail and mix immediately. 3. Incubate at 25°C for 20 minutes. 4. Record the increase in A650nm for 15 minutes., 63mM sodium phosphate, Protocol: Activity Assay 1. Prepare a 1 ml reaction cocktail into a suitable container : The final concentrations are 5mM Tris |
Reviews
Documents
| FAQs |
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Resources
| Recombinant Protein Resources |
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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