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Peroxiredoxin-1 / PRDX1 (1-199, His-Tag) Human Protein

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SKU
AR09077PU-N
Recombinant human Peroxiredoxin 1/PRDX1 protein
Size:
100 ug
  $455.00
3 Weeks*
Bulk/Customize

Other products for "PRDX1 or NM_001202431"

Specifications
Specifications
Product Data
Species Human
Expression Host E. coli
Expression cDNA Clone or AA Sequence
Protein Sequence
MSSGNAKIGH PAPNFKATAV MPDGQFKDIS LSDYKGKYVV FFFYPLDFTF VCPTEIIAFS DRAEEFKKLN CQVIGASVDS HFCHLAWVNT PKKQGGLGPM NIPLVSDPKR TIAQDYGVLK ADEGISFRGL FIIDDKGILR QITVNDLPVG RSVDETLRLV QAFQFTDKHG EVCPAGWKPG SDTIKPDVQK SKEYFSKQK
Tag His-Tag
Predicted MW 24 kDa (219aa) confirmed by MALDI-TOF
Concentration 1mg/ml (determined by Bradford assay)
Purity > 90% by SDS-PAGE
Presentation State Purified
Buffer Liquid in. 20mM Tris-HCl buffer (pH 7.5) containing 20% glycerol
Bioactivity Specific activity is >2,000pmol/min/ug. Enzymatic activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25C for minute.
Preparation Liquid purified protein
Recommended Dilution SDS-PAGE, Enzyme Activity
Protein Description Recombinant Human PRDX1, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography techniques.
Storage Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Reference Data
RefSeq NP_002565.1
Locus ID 5052
UniProt ID Q06830
Cytogenetics 1p34.1
Synonyms Natural killer cell-enhancing factor A, NKEF-A, Proliferation-associated gene protein, PAG, Thioredoxin peroxidase 2, Thioredoxin-dependent peroxide reductase 2, Thioredoxin-dependent peroxiredoxin 1, PAGA, PAGB, TDPX2
Summary This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. Four transcript variants encoding the same protein have been identified for this gene. provided by RefSeq, Jan 2011
Protein Categories Intracellular Proteins
Protein Families 0.03X PBS, 0.5% glycerol.
2. Add 5 µl of recombinant PRDX1 solution with various concentrations (0.25 µg, 2.5M KSCN)
7. Record the increase in A475nm., Protocol: Activity Assay:
1. Prepare a 50 µl reaction mix into a suitable container :
The final concentrations are 1mM DTT
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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