Cyclophilin H / PPIH (1-177) Human Protein

SKU
AR09072PU-N
Recombinant human Cyclophilin H/PPIH protein
  $570.00
3 Weeks*
Bulk/Customize
Specifications
Specifications
Product Data
Species Human
Expression Host E. coli
Expression cDNA Clone or AA Sequence
Protein Sequence
MAVANSSPVN PVVFFDVSIG GQEVGRMKIE LFADVVPKTA ENFRQFCTGE FRKDGVPIGY KGSTFHRVIK DFMIQGGDFV NGDGTGVASI YRGPFADENF KLRHSAPGLL SMANSGPSTN GCQFFITCSK CDWLDGKHVV FGKIIDGLLV MRKIENVPTG PNNKPKLPVV ISQCGEM
Predicted MW 19.2 kDa (177aa) confirmed by MALDI-TOF
Concentration 1mg/ml (determined by Bradford assay)
Purity > 95% by SDS-PAGE
Presentation State Purified
Buffer Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Bioactivity Specific activity is > 220nmol/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAPF-pNA per minute at 37C in Tris-Hcl pH8.0 using chymotrypsin.
Endotoxin < 1 EU per 1ug of protein (determined by LAL method)
Preparation Liquid purified protein
Recommended Dilution SDS-PAGE, Enzyme Activity
Protein Description Recombinant human cyclophilin H was expressed in E.coli and purified by conventional chromatography techniques.
Storage Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Reference Data
RefSeq NP_006338
Locus ID 10465
UniProt ID O43447
Cytogenetics 1p34.2
Synonyms Peptidylprolyl isomerase H, Peptidylprolyl isomerase H, PPIH, CYPH, CYP20, SnuCyp-20, SnuCyp-20, PPIase H, Rotamase H, Cyclophilin H, Peptidylprolyl isomerase H CypH, EC 5.2.1.8, MGC5016, Small nuclear ribonucleoprotein particle-specific cyclophilin H, uSACYP, usnRNP associated cyclophilin SnuCyp20
Summary The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is a specific component of the complex that includes pre-mRNA processing factors PRPF3, PRPF4, and PRPF18, as well as U4/U5/U6 tri-snRNP. This protein has been shown to possess PPIase activity and may act as a protein chaperone that mediates the interactions between different proteins inside the spliceosome. provided by RefSeq, Jul 2008
Protein Categories Intracellular Proteins
Protein Families pH 8.0, Protocol: Activity Assay
1. Prepare 170 ul assay buffer into a suitable container and pre-chill on ice before use:
The final concentrations are 200 mM Tris-HCl
Protein Pathways Spliceosome
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