nusA / L-factor (1-495) E.coli Protein
Specifications
| Product Data | |
| Species | E.coli |
|---|---|
| Expression Host | E. coli |
| Expression cDNA Clone or AA Sequence | |
| Predicted MW | 54 kDa (495aa) confirmed by MALDI-TOF |
| Concentration | 1mg/ml (determined by Bradford assay) |
| Purity | > 95% by SDS-PAGE |
| Presentation State | Purified |
| Buffer | Liquid in. Phosphate-Buffered Saline (pH 7.4) |
| Preparation | Liquid purified protein |
| Recommended Dilution | SDS-PAGE |
| Protein Description | Recombinant NusA was expressed in E.coli and purified by using conventional chromatography techniques. |
| Storage | Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Reference Data | |
| RefSeq | AAC76203 |
| UniProt ID | P0AFF6 |
| Synonyms | Transcription termination/antitermination L factor, ECK3158, JW3158, Transcription termination/antitermination L factor, NusA, Transcription termination/antitermination L factor |
| Summary | NusA is a key component in both Prevention and enhancement of transcriptional termination. It is important in both Rho-dependent and intrinsic termination, as well as in lambda and other phage antitermination systems. The gene was first identified by isolation of the nusAl mutation, which restricts bacteriophage l growth by preventing the antitermination activity of the l N protein. NusA is involved in transcriptional antitermination in the cell. It has been shown to specifically aid in read-through of the RNA polymerase genes rpoB and rpoC, as well as in successful synthesis of the ribosomal RNA genes. Recombinant NusA was expressed in E. coli and purified by using conventional chromatography techniques |
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