Neuron specific enolase (1-434) Human Protein

SKU
AR09061PU-L
Recombinant human Enolase 2/ENO2 protein
  $1,135.00
3 Weeks*
Bulk/Customize
Specifications
Specifications
Product Data
Species Human
Expression Host E. coli
Expression cDNA Clone or AA Sequence
Protein Sequence
MSIEKIWARE ILDSRGNPTV EVDLYTAKGL FRAAVPSGAS TGIYEALELR DGDKQRYLGK GVLKAVDHIN STIAPALISS GLSVVEQEKL DNLMLELDGT ENKSKFGANA ILGVSLAVCK AGAAERELPL YRHIAQLAGN SDLILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAESFRD AMRLGAEVYH TLKGVIKDKY GKDATNVGDE GGFAPNILEN SEALELVKEA IDKAGYTEKI VIGMDVAASE FYRDGKYDLD FKSPTDPSRY ITGDQLGALY QDFVRDYPVV SIEDPFDQDD WAAWSKFTAN VGIQIVGDDL TVTNPKRIER AVEEKACNCL LLKVNQIGSV TEAIQACKLA QENGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL MRIEEELGDE ARFAGHNFRN PSVL
Predicted MW 47.2 kDa ( 434aa) confirmed by MALDI-TOF
Concentration 1mg/ml (determined by Bradford assay)
Purity > 95% by SDS-PAGE
Presentation State Purified
Buffer Liquid in. 20mM Tris-HCl buffer (pH 7.5) containing 0.1 M KCl, 5mM MgSO4
Bioactivity Specific activity is > 25,000pmol/min/ug, and was obtained by measuring the decrease of NAD in absorbance at 340nm resulting from NADH at pH 6.5 at 37C.
Endotoxin < 1 EU per 1ug of protein (determined by LAL method)
Preparation Liquid purified protein
Recommended Dilution SDS-PAGE, Enzyme Activity
Protein Description Recombinant NSE was expressed in E.coli and purified by conventional chromatography techniques.
Storage Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Reference Data
RefSeq NP_001966.1
Locus ID 2026
UniProt ID P09104
Cytogenetics 12p13.31
Synonyms Enolase 2 (gamma, neuronal), ENO2, NSE, Neuron-Specific Enolase, 2 phospho D glycerate hydrolyase, Eno 2, ENOG, Enolase 2 gamma neuronal, Enolase2, Gamma enolase, Neural enolase, Neuron specific enolase, Neuron specific gamma enolase, Neurone specific enolase.
Summary This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. provided by RefSeq, Jul 2008
Protein Categories Intracellular Proteins
Protein Pathways Glycolysis / Gluconeogenesis, Metabolic pathways, RNA degradation
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