Interleukin-2 / IL2 (C145S) Human Protein

CAT#: AR09046PU-N

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Interleukin-2 / IL2 (C145S) human recombinant protein, 0.1 mg



USD 415.00


Availability*
2 Weeks

Size
    • 100 ug


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Specifications

Product Data
Description Interleukin-2 / IL2 (C145S) human recombinant protein, 0.1 mg
Species Human
Expression Host E. coli
Expression cDNA Clone or AA Sequence MPTSSSTKKT QLQLEHLLLD LQMILNGINN YKNPKLTRML TFKFYMPKKA TELKHLQCLE EELKPLEEVL NLAQSKNFHL RPRDLISNIN VIVLELKGSE TTFMCEYADE TATIVEFLNR WITFSQSIIS TLT
Predicted MW 15.4 kDa
Concentration 0.5 mg/ml (determined by Bradford assay)
Purity >95% by SDS PAGE
Buffer Presentation State: Purified
State: Liquid purified protein
Buffer System: PBS, pH 7.4
Bioactivity Biological: Measured in a cell proliferation assay using CTLL2
mouse cytotoxic T cells. The ED50 for this effect is < 1.5 ng/ml.
Activity Assay
1. Cell line: CTLL2 (mouse cytotoxic T cell)
2. Maintenance Condition: 10% FBS RPMI 1640 with hIL2
3. Assay medium: 10% FBS RPMI 1640 without hIL2
4. Cell Density: 2 x 10e4 cells/well (96 well plate, final volume 100ul)
5. Starvation : 24hr, Assay medium
6. Incubation Time : 24hr (after sample treatment)
7. Concentration Range: 0.039 ng/ml - 40 ng/ml
8. Detection method : MTT assay
Preparation Liquid purified protein
Protein Description This recombinant human IL-2 is a 15.4 kDa protein, containing 133 amino acid residues including one intra chain disulfide bond. Recombinant human IL-2 was overexpressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Storage Store (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Reference Data
RefSeq NP_000577
Locus ID 3558
Cytogenetics 4q27
Synonyms IL-2; lymphokine; TCGF
Summary The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq, Jul 2008]
Protein Families Druggable Genome, Secreted Protein
Protein Pathways Allograft rejection, Autoimmune thyroid disease, Cytokine-cytokine receptor interaction, Graft-versus-host disease, Jak-STAT signaling pathway, T cell receptor signaling pathway, Type I diabetes mellitus

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