Interleukin-2 / IL2 (22-153) Human Protein
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | E. coli |
| Expression cDNA Clone or AA Sequence | |
| Predicted MW | 15.4 kDa (133aa) confirmed by MALDI-TOF |
| Concentration | 0.5mg/ml (determined by Bradford assay) |
| Purity | > 95% by SDS-PAGE |
| Presentation State | Purified |
| Buffer | Liquid in. Phosphate-Buffered Saline (pH 7.4) |
| Bioactivity | Measured in a cell proliferation assay using CTLL2 mouse cytotoxic T cells. The ED50 range ≤0.65ng/ml. |
| Endotoxin | < 1 EU per 1ug of protein (determined by LAL method) |
| Preparation | Liquid purified protein |
| Recommended Dilution | SDS-PAGE, Bioactivity |
| Protein Description | This recombinant human IL-2 is a 15.4 kDa protein, containing 133 amino acid residues including one intra chain disulfide bond. Recombinant human IL-2 was overexpressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. |
| Storage | Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Reference Data | |
| RefSeq | NP_000577.2 |
| Locus ID | 3558 |
| UniProt ID | P60568 |
| Cytogenetics | 4q27 |
| Synonyms | IL-2, TCGF, Aldesleukin, T-cell growth factor, Interleukin-2 |
| Summary | This gene is a member of the interleukin 2 (IL2) cytokine subfamily which includes IL4, IL7, IL9, IL15, IL21, erythropoietin, and thrombopoietin. The protein encoded by this gene is a secreted cytokine produced by activated CD4+ and CD8+ T lymphocytes, that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2R) is a heterotrimeric protein complex whose gamma chain is also shared by IL4 and IL7. The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. provided by RefSeq, Sep 2020 |
| Protein Categories | Allergies and autoimmune diseases, Cytokines, Diabetes, Growth Factors, Secreated Proteins |
| Protein Families | Druggable Genome, Secreted Protein |
| Protein Pathways | Allograft rejection, Autoimmune thyroid disease, Cytokine-cytokine receptor interaction, Graft-versus-host disease, Jak-STAT signaling pathway, T cell receptor signaling pathway, Type I diabetes mellitus |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Citations
MAC-T Cells as a Tool to Evaluate Lentiviral Vector Construction Targeting Recombinant Protein Expression in Milk
Monzani, Guemra, Adona et al
Anim. Biotechnol. (2015) 26 (2), 136-42 DOI: 10.1080/10495398.2014.941468
Monzani, Guemra, Adona et al
Anim. Biotechnol. (2015) 26 (2), 136-42 DOI: 10.1080/10495398.2014.941468
Cited by 9
Showing 1-1 of 1 papers.
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