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grpE / HSP24 (1-197) E.coli Protein

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SKU
AR09033PU-L
Recombinant e.coli GrpE protein (V44I)
Size:
500 ug
  $1,135.00
3 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species E.coli
Expression Host E. coli
Expression cDNA Clone or AA Sequence
Protein Sequence
MSSKEQKTPE GQAPEEIIMD QHEEIEAVEP EASAEQVDPR DEKIANLEAQ LAEAQTRERD GILRVKAEME NLRRRTELDI EKAHKFALEK FINELLPVID SLDRALEVAD KANPDMSAMV EGIELTLKSM LDVVRKFGVE VIAETNVPLD PNVHQAIAMV ESDDVAPGNV LGIMQKGYTL NGRTIRAAMV TVAKAKA
Predicted MW 21.8 kDa (197aa) confirmed by MALDI-TOF
Concentration 1mg/ml (determined by Bradford assay)
Purity > 90% by SDS-PAGE
Presentation State Purified
Buffer Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 100mM NaCl
Preparation Liquid purified protein
Recommended Dilution SDS-PAGE
Protein Description Recombinant e.coli GrpE protein (V44I) was overexpressed in E.coli and purified by using the conventional column chromatography techniques.
Note NCBI Accession number: NP_417104
Storage Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Reference Data
RefSeq NP_417104.1
UniProt ID P09372
Synonyms HSP-70 cofactor, Heat shock protein B25.3, HSP24, GrpE, HSP 70 cofactor.
Summary GrpE, co-chaperone of E. coli, participates actively in the response to hyperosomotic and heat shock by preventing the aggregation of stress-denatured proteins in association with DnaK. This protein is the nucleotide exchange factor for DnaK and may function as a thermosensor. Several rounds of ATP-dependent interactions between DnaJ, Dna K and GrpE are required for fully efficient folding. Recombinant GrpE protein was overexpressed in E. coli and purified by using the conventional column chromatography techniques.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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