DMG (N,N-dimethylglycine oxidase) (1-830, His-Tag) Arthrobacter globiformis Protein
Specifications
| Product Data | |
| Species | Arthrobacter globiformis |
|---|---|
| Expression Host | E. coli |
| Expression cDNA Clone or AA Sequence | |
| Tag | His-Tag |
| Predicted MW | 92.1 kDa (850aa) confirmed by MALDI-TOF |
| Concentration | 1mg/ml (determined by Bradford assay) |
| Purity | > 95% by SDS-PAGE |
| Presentation State | Purified |
| Buffer | Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol |
| Preparation | Liquid purified protein |
| Recommended Dilution | SDS-PAGE |
| Protein Description | Recombinant DMGO originated from Arthrobacter globifomis, fused to His tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Storage | Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Reference Data | |
| RefSeq | AF329477 |
| UniProt ID | Q9AGP8 |
| Synonyms | Dimethylglycine oxidase, DMG |
| Summary | Dimethylglycine oxidase (DMGO) is a covalent flavoenzyme from Arthrobacter globiformis that catalyzes the oxidative demethylation of dimethylglycine to yield sarcosine, formaldehyde, and hydrogen peroxide. The N-terminal region binds FAD covalently so it is yellowish. Recombinant DMGO originated from Arthrobacter globifomis, fused to His tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. |
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