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AKT1 / PKB

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SKU
AR08011PU-N
AKT/PKBa Substrate
Size:
500 ug
Conjugation:
Unconjugated
  $485.00
5 Days*
Bulk/Customize
Specifications
Recommended Dilution WB: 1:500 - 1:2,000
Application WB
Background Akt is a serine/threonine kinase that enhances cell proliferation and inhibits apoptosis when activated. Akt associates with PI(3,4,5)P3) at the cell membrane through its pleckstrin homology domain and is activated through phosphorylation. 3-Phosphoinositide-dependent kinase-1 (PDK1) has been shown to first phosphorylate Akt at Thr308, then a second kinase phosphorylates Akt at position Ser473. In turn, Akt phosphorylates a host of proteins including the proapoptotic proteins BAD and pro-caspase-9, GSK3, p21WAF1, MDM2, and the forkhead (FKHR) family of transcription factors.
Clonality Polyclonal
Concentration 1.0 mg/mL - lot specific
Conjugation Unconjugated
Mutant No
Note AKT/PKBa Substrate control peptide should be used at 1.0 µg per 1.0 µl of antiserum in per assay.
Reconstitution Method Restore with deionized water (or equivalent) - Reconstitution Volume: 500 µL
Specificity Greater than 98% specific peptide by HPLC analysis
Stability Expiration date is six (6) months from date of receipt.
Storage Store vial at 2 - 8 ° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
Synonyms AKT1, serine/threonine kinase, apoptosis inhibitor substrate peptides, blocking peptide, control peptide
OTI product type code control-peptide
OTI production type 2
Datasheet URL /datasheet/ar08011pu-n.pdf
Image Captions []
SDS URL sds/ar08011pu-n.pdf
Size 500 ug
Product Type Antibodies
Product Type Control Peptides
PDB Internal ID 1889376
Target Symbol AKT1
Availability 5 Days
New No
Shipping Ambient
Keywords AKT; C-AKT; MGC99656; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA; RAC-PK-alpha
Has Matched ELISA Pair No
Base SKU AR08011PU-N
Target Symbol AKT1
Short Description AKT/PKBa Substrate
Description AKT/PKBa Substrate
URL Key ar08011pu-n-akt1-pkb
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Documents
Resources

file-alt Citations

Identification of AKT1/β-catenin mutations conferring cetuximab and chemotherapeutic drug resistance in colorectal cancer treatment
Hasbal-Celikok, Aksoy-Sagirli, Altiparmak-Ulbegi et al
Oncol Lett (2021) 21 (3), 209 DOI: 10.3892/ol.2021.12470
Cited by 25
AI Summary: In this study, OriGene products—including the AKT1 (RC213307) and CTNNB1 (RC210744) expression plasmids—were used to generate mutant and wild-type constructs for transfection experiments, enabling the authors to investigate how specific AKT1 and β-catenin mutations contribute to drug resistance in colorectal cancer cells.
Cytotoxic Amphiphiles and Phosphoinositides Bind to Two Discrete Sites on the Akt1 PH Domain
Gradziel, Wang, Stec et al
Biochemistry (2014) 53 (3), 462-472 DOI: 10.1021/bi401720v
Cited by 10
AI Summary: OriGene contributed human Akt1 cDNA, which was used for cloning, overexpression, and purification of Akt1 His6-PH and mutated proteins in this study.
Snippet: Research Chemicals. Other chemicals used were obtained from Sigma-Aldrich. Cloning, Overexpression, and Purification of Akt1 His 6 -PH and Mutated Proteins Human Akt1 cDNA was obtained from Origene. Polymerase chain ... Cite SaveSaving
Discovery of a novel class of AKT pleckstrin homology domain inhibitors
Mahadevan, Powis, Mash et al
Mol Cancer Ther (2008) 7 (9), 2621-32 DOI: 10.1158/1535-7163.MCT-07-2276
AI Summary: In this study, OriGene products—including the human AKT1 (NM_005163) cDNA clone—were used to express and analyze AKT1, enabling the evaluation of novel PH domain inhibitors' selectivity and efficacy.
FigureFigure
Showing 1-3 of 3 papers.
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