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Superoxide Dismutase 1 / SOD1 (1-154) Human Protein

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SKU
AR03033PU-L
Recombinant human SOD1/Cu-Zn SOD protein
Size:
500 ug
  $1,135.00
3 Weeks*
Bulk/Customize

Other products for "SOD1 or NM_000454"

Specifications
Specifications
Product Data
Species Human
Expression Host E. coli
Expression cDNA Clone or AA Sequence
Protein Sequence
MATKAVCVLK GDGPVQGIIN FEQKESNGPV KVWGSIKGLT EGLHGFHVHE FGDNTAGCTS AGPHFNPLSR KHGGPKDEER HVGDLGNVTA DKDGVADVSI EDSVISLSGD HCIIGRTLVV HEKADDLGKG GNEESTKTGN AGSRLACGVI GIAQ
Predicted MW 15.9 kDa (154aa) confirmed by MALDI-TOF
Concentration 1mg/ml (determined by Bradford assay)
Purity > 95% by SDS-PAGE
Presentation State Purified
Buffer Liquid in. 20mM Tris-HCl buffer (pH 7.5) containing 10% glycerol
Bioactivity Specific activity is > 500unit/mg, in which one unit will inhibit the rate of reduction of cytochrome c by 50% in a coupled system, using xanthine and Xanthine oxidase at pH 7.5 at 25C.
Endotoxin < 1 EU per 1ug of protein (determined by LAL method)
Preparation Liquid purified protein
Recommended Dilution SDS-PAGE, Enzyme Activity
Protein Description Recombinant SOD1 was expressed in E.coli and purified by conventional chromatography techniques.
Storage Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Reference Data
RefSeq NP_000445.1
Locus ID 6647
UniProt ID P00441
Cytogenetics 21q22.11
Synonyms Superoxide dismutase 1 soluble, ALS, SOD, ALS1, IPOA, Cu-Zn superoxide dismutase, Superoxide dismutase 1 soluble, SOD1, Superoxide dismutase 1, soluble ALS 1, Amyotrophic lateral sclerosis 1 Amyotrophic lateral sclerosis 1 adult, Cu/Zn SOD, Cu/Zn superoxide dismutase, Homodimer, Indophenoloxidase A, Mn superoxide dismutase, SOD 1, SOD soluble, SOD2, SODC, Superoxide dismutase 1 soluble, Superoxide dismutase Cu Zn, Superoxide dismutase cystolic
Summary The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. In addition, this protein contains an antimicrobial peptide that displays antibacterial, antifungal, and anti-MRSA activity against E. coli, E. faecalis, S. aureus, S. aureus MRSA LPV+, S. agalactiae, and yeast C. krusei. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. provided by RefSeq, Jul 2020
Protein Categories Cytokines, Intracellular Proteins, Nervous system: Neurodegenerative diseases
Protein Families 0.005 units xanthine oxidase.
2. Equilibrate to 25°C and monitor at A550nm until the value is constant using a spectrophotometer.
3. Add 50 ul of recombinant SOD protein in various concentrations (0.5ug, 0.01 mM cytochrom C, 0.05 mM xanthine, 0.1 mM ethylendiaminetetraacetic acid, 1ug) in assay buffer.
4. Mix by inversion and record the increase at A550nm for 5 minutes., Protocol: Activity Assay
1. Prepare a 1.5 ml reaction mix into a suitable container and pre-chill on ice before use: The final concentrations are 50 mM potassium phosphate
Protein Pathways Amyotrophic lateral sclerosis (ALS), Huntington's disease, Prion diseases
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Documents
Resources

file-alt Citations

Candesartan Normalizes Changes in Retinal Blood Flow and p22phox in the Diabetic Rat Retina
Eshaq, Watts, Carter et al
Pathophysiology (2021) 28 (1), 86-97 DOI: 10.3390/pathophysiology28010008
Cited by 3
AI Summary: OriGene products contributed to this study by providing the p22phox antibody (OriGene, Cat# TA326733), which was used to assess changes in p22phox protein levels in the diabetic rat retina.
FigureFigureFigure
Housefly (Musca domestica) larvae powder, preventing oxidative stress injury via regulation of UCP4 and CyclinD1 and modulation of JNK and P38 signaling in APP/PS1 mice
He, Yang, Jiao et al
Food Funct (2019) 10 (1), 235-243 DOI: 10.1039/c8fo02052c
Cited by 7
AI Summary: In this study, OriGene products—specifically the anti-UCP4 antibody (TA326085), anti-CyclinD1 antibody (TA500249), anti-SOD1 antibody (TA500053), and anti-CAT antibody (TA352768)—were used for immunohistochemistry and western blot analyses to assess protein expression levels in APP/PS1 mice, thereby enabling evaluation of the protective effects of housefly larvae powder against oxidative stress and memory impairment.
The dual roles of c-Jun NH2-terminal kinase signaling in Cr(VI)-induced apoptosis in JB6 cells
Son, Hitron, Cheng et al
Toxicol. Sci. (2011) 119 (2), 335-45 DOI: 10.1093/toxsci/kfq335
Cited by 14
AI Summary: OriGene products, specifically the SOD1-Myc-DDK and SOD2-enhanced green fluorescent protein–tagged plasmids, were used in this study to investigate the roles of superoxide dismutase in Cr(VI)-induced apoptosis and JNK signaling in JB6 cells.
Snippet: The SOD1-Myc-DDK– and SOD2-enhanced green fluorescent protein–tagged plasmids were purchased from Origene (Rockville, MD) and Addgene ... Figure 5 shows that knocking down c-Jun expression by si-RNA transfection significantly enhances cytotoxicity and apoptosis in
Showing 1-3 of 3 papers.
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