HSPA5 / GRP78 (Active Protein) Human Protein

CAT#: AR03021PU-N

HSPA5 / GRP78 (Active Protein) human recombinant protein, 0.1 mg

Product Images

SDS-PAGE of 78kDa Grp78 (Bip) protein (AR03021PU).

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Concentration: lot specific
• Purity: >90% pure as determined by SDS-PAGE analysis
• Presentation: Purified
• Buffer: Presentation State: Purified
  State: Liquid purified protein
  Buffer System: 50 mM Tris/HCl, pH 7.5, 150 mM NaCl and 10% Glycerol
• Preparation: Liquid purified protein
• Applications: Western Blot Control.
  ATPase activity.
  This protein has ATPase activity at the time of manufacture of 2.3 µM Phosphate liberated/hr/µg protein in a 200 µl reaction at 37°C (pH 8) in the presence of 20 µl of 1mM ATP using Malachite Green Assay.
  Binding Assay.
  ELISA reference standard.
• Protein Description: Recombinant Human GRP78 Protein with ATPase activity, His-tagged.
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_005338
• Locus ID: 3309
• UniProt ID: P11021, V9HWB4
• Cytogenetics: 9q33.3
• Synonyms: BIP; GRP78; HEL-S-89n
• Summary: The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. This protein localizes to the lumen of the endoplasmic reticulum (ER) where it operates as a typical HSP70 chaperone involved in the folding and assembly of proteins in the ER and is a master regulator of ER homeostasis. During cellular stress, as during viral infection or tumorogenesis, this protein interacts with the transmembrane stress sensor proteins PERK (protein kinase R-like endoplasmic reticulum kinase), IRE1 (inositol-requiring kinase 1), and ATF6 (activating transcription factor 6) where it acts as a repressor of the unfolded protein response (UPR) and also plays a role in cellular apoptosis and senescence. Elevated expression and atypical translocation of this protein to the cell surface has been reported in viral infections and some types of cancer cells. At the cell surface this protein may facilitate viral attachment and entry to host cells. This gene is a therapeutic target for the treatment of coronavirus diseases and chemoresistant cancers. [provided by RefSeq, Jul 2020]
• Protein Families: Druggable Genome
• Protein Pathways: Antigen processing and presentation, Prion diseases