KRAS (NM_004985) Mutant (G12D) Human Recombinant Protein
SKU
TP700052
KRAS mutant (G12D) Myc-DDK-tagged human recombinant protein of Homo sapiens v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant b
$900.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Myc/DDK |
| Predicted MW | 21.2 kDa |
| Concentration | >0.1 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Bioactivity | MS digestion standard (PMID: 29203663) |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_004976 |
| Locus ID | 3845 |
| Cytogenetics | 12p12.1 |
| RefSeq Size | 5312 |
| RefSeq ORF | 564 |
| Synonyms | C-K-RAS; c-Ki-ras2; CFC2; K-Ras; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KI-RAS; KRAS1; KRAS2; NS; NS3; RALD; RASK2 |
| Summary | This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. provided by RefSeq, Jul 2008 |
| Protein Categories | ADC Targets, Cancer: Endocrine, Cancer: Gastrointestinal, Cancer: Gynecologic, Cancer: Haematopoietic and Lymphoid, Cancer: Head and Neck, Cancer: Lung, Cancer: Sarcoma, Cancer: Skin, Intracellular Proteins |
| Protein Families | Druggable Genome |
| Protein Pathways | Acute myeloid leukemia, Axon guidance, B cell receptor signaling pathway, Bladder cancer, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Dorso-ventral axis formation, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Gap junction, Glioma, GnRH signaling pathway, Insulin signaling pathway, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Melanoma, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway, Thyroid cancer, Tight junction, VEGF signaling pathway |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "KRAS" proteins (8)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH301958 | KRAS MS Standard C13 and N15-labeled recombinant protein (NP_004976) | 10 ug |
$3,360.00
|
|
| PH322697 | KRAS MS Standard C13 and N15-labeled recombinant protein (NP_203524) | 10 ug |
$3,360.00
|
|
| LC409579 | Lyophilized KRAS HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LC417614 | Lyophilized KRAS HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY409579 | Transient overexpression lysate of v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant a (as WB positive control) | 100 ug |
$436.00
|
|
| LY417614 | Transient overexpression lysate of v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant b (as WB positive control) | 100 ug |
$436.00
|
|
| TP301958 | Recombinant protein of human v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant b, 20 µg | 20 ug |
$867.00
|
|
| TP322697 | Recombinant protein of human v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant a, 20 µg | 20 ug |
$867.00
|
|
Citations
The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2
Kovalski, Bhaduri, Zehnder et al
Mol. Cell (2019) 73 (4), 830-844.e12 DOI: 10.1016/j.molcel.2018.12.001
Kovalski, Bhaduri, Zehnder et al
Mol. Cell (2019) 73 (4), 830-844.e12 DOI: 10.1016/j.molcel.2018.12.001
AI Summary: In this study, OriGene’s recombinant proteins—including NRASQ61K (Cat# TP701004), NRAS (Cat# TP302681), MAPKAP1 (Cat# TP311745), mTOR (Cat# TP320457), KRASG12D (Cat# TP700052), HRASWT (Cat# TP316409), and custom-produced HRASG12V—were used to investigate and validate the interactions between oncogenic Ras proteins and mTORC2 components, elucidating key mechanisms in Ras-driven cancer proliferation.
Snippet: … They demonstrate that active Ras binds and stimulates mTORC2, representing a key dependency for Ras-driven proliferation genes and tumorigenesis. Cancer Ras Proteomics CRISPR mTOR MAPKAP1;... … They demonstrate that active Ras binds and stimulates mTORC2, representing a key dependency for Ras-driven proliferation genes and tumorigenesis. Cancer Ras Proteomics CRISPR mTOR MAPKAP1;NRASQ61K Human Recombinant Protein Origene Cat# TP701004 NRAS Human Recombinant Protein Origene Cat# TP302681 MAPKAP1 Human Recombinant Protein Origene Cat# TP311745 mTOR Human Recombinant Protein Origene Cat# TP320457 KRASG12D Human Recombinant Protein Origene Cat# TP700052 HRASWT Human Recombinant Protein Origene Cat# TP316409 HRASG12V Human Recombinant Protein Origene Custom Production from clone Cat# RC400129
Selected reaction monitoring approach for validating peptide biomarkers
Wang, Zhang, Tomita et al
PNAS (2017) 114 (51), 13519-13524 DOI: 10.1073/pnas.1712731114
Wang, Zhang, Tomita et al
PNAS (2017) 114 (51), 13519-13524 DOI: 10.1073/pnas.1712731114
AI Summary: OriGene’s heavy-isotope–labeled K-Ras mutant (G12D) protein was used as an internal standard to normalize peptide abundances during sample preparation, ensuring accurate validation of candidate peptide biomarkers in this study.
Snippet: Variations in sample preparation were adjusted by normalizing the abundance of each peptide from a given sample to the abundance of the peptides derived from the heavy-isotope–labeled K-Ras mutant (G12D) protein purchased from Origene
Showing 1-2 of 2 papers.
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