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EEA1 (NM_003566) Human Mass Spec Standard

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SKU
PH315396
EEA1 MS Standard C13 and N15-labeled recombinant protein (NP_003557)
Size:
10 ug
  $3,360.00
In Stock*
Bulk/Customize

Frequently Bought Together (2)

TA813373 EEA1 mouse monoclonal antibody, clone OTI2G7 100 ul
$447.00
LY418586 Transient overexpression lysate of early endosome antigen 1 (EEA1) (as WB positive control) 100 ug
$665.00

Other products for "EEA1 or NM_003566"

Specifications
Specifications
Product Data
Tag C-Myc/DDK
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence RC215396
Predicted MW 162.3 kDa
Protein Sequence
Protein Sequence
>RC215396 protein sequence
Red=Cloning site Green=Tags(s)

MLRRILQRTPGRVGSQGSDLDSSATPINTVDVNNESSSEGFICPQCMKSLGSADELFKHYEAVHDAGNDS
GHGGESNLALKRDDVTLLRQEVQDLQASLKEEKWYSEELKKELEKYQGLQQQEAKPDGLVTDSSAELQSL
EQQLEEAQTENFNIKQMKDLFEQKAAQLATEIADIKSKYDEERSLREAAEQKVTRLTEELNKEATVIQDL
KTELLQRPGIEDVAVLKKELVQVQTLMDNMTLERERESEKLKDECKKLQSQYASSEATISQLRSELAKGP
QEVAVYVQELQKLKSSVNELTQKNQTLTENLLKKEQDYTKLEEKHNEESVSKKNIQATLHQKDLDCQQLQ
SRLSASETSLHRIHVELSEKGEATQKLKEELSEVETKYQHLKAEFKQLQQQREEKEQHGLQLQSEINQLH
SKLLETERQLGEAHGRLKEQRQLSSEKLMDKEQQVADLQLKLSRLEEQLKEKVTNSTELQHQLDKTKQQH
QEQQALQQSTTAKLREAQNDLEQVLRQIGDKDQKIQNLEALLQKSKENISLLEKEREDLYAKIQAGEGET
AVLNQLQEKNHTLQEQVTQLTEKLKNQSESHKQAQENLHDQVQEQKAHLRAAQDRVLSLETSVNELNSQL
NESKEKVSQLDIQIKAKTELLLSAEAAKTAQRADLQNHLDTAQNALQDKQQELNKITTQLDQVTAKLQDK
QEHCSQLESHLKEYKEKYLSLEQKTEELEGQIKKLEADSLEVKASKEQALQDLQQQRQLNTDLELRATEL
SKQLEMEKEIVSSTRLDLQKKSEALESIKQKLTKQEEEKQILKQDFETLSQETKIQHEELNNRIQTTVTE
LQKVKMEKEALMTELSTVKDKLSKVSDSLKNSKSEFEKENQKGKAAILDLEKTCKELKHQLQVQMENTLK
EQKELKKSLEKEKEASHQLKLELNSMQEQLIQAQNTLKQNEKEEQQLQGNINELKQSSEQKKKQIEALQG
ELKIAVLQKTELENKLQQQLTQAAQELAAEKEKISVLQNNYEKSQETFKQLQSDFYGRESELLATRQDLK
SVEEKLSLAQEDLISNRNQIGNQNKLIQELKTAKATLEQDSAKKEQQLQERCKALQDIQKEKSLKEKELV
NEKSKLAEIEEIKCRQEKEITKLNEELKSHKLESIKEITNLKDAKQLLIQQKLELQGKADSLKAAVEQEK
RNQQILKDQVKKEEEELKKEFIEKEAKLHSEIKEKEVGMKKHEENEAKLTMQITALNENLGTVKKEWQSS
QRRVSELEKQTDDLRGEIAVLEATVQNNQDERRALLERCLKGEGEIEKLQTKVLELQRKLDNTTAAVQEL
GRENQSLQIKHTQALNRKWAEDNEVQNCMACGKGFSVTVRRHHCRQCGNIFCAECSAKNALTPSSKKPVR
VCDACFNDLQG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration >0.05 µg/µL as determined by microplate BCA method
Labeling Method Labeled with U- 13C6, 15N4-L-Arginine and U- 13C6, 15N2-L-Lysine
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3
Storage Store at -80°C. Avoid repeated freeze-thaw cycles.
Stability Stable for 3 months from receipt of products under proper storage and handling conditions.
Shipping Dry Ice
Reference Data
RefSeq NP_003557
RefSeq Size 4874
RefSeq ORF 4233
Synonyms MST105; MSTP105; ZFYVE2
Locus ID 8411
UniProt ID Q15075
Cytogenetics 12q22
Summary Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.UniProtKB/Swiss-Prot Function
Protein Pathways Endocytosis
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Other "EEA1" proteins (3)
SKU Description Size Price
LC418586 Lyophilized EEA1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY418586 Transient overexpression lysate of early endosome antigen 1 (EEA1) (as WB positive control) 100 ug
$665.00
TP315396 Recombinant protein of human early endosome antigen 1 (EEA1), 20 µg 20 ug
$737.00

file-alt Citations

Monoubiquitination of EEA1 regulates endosome fusion and trafficking
Ramanathan, Zhang, Ye
Cell Biosci (2013) 3 (1), 24 DOI: 10.1186/2045-3701-3-24
Cited by 30
AI Summary: OriGene's full-length human EEA1 protein was used in this study to investigate how monoubiquitination regulates EEA1 function in endosome fusion and trafficking.
Snippet: Full length human EEA1 was purchased (OriGene). ... Rabbit anti-EEA1 (Cell Signaling), rabbit anti-FLAG (Sigma);mouse anti-EEA1 (BD Biosciences);rabbit anti-β-COP (Thermo Scientific, IL), rabbit PDI (Enzo life sciences, NY), mouse Tom20 (clone F10, Santa Cruz
FigureFigureFigure
The p97 ATPase associates with EEA1 to regulate the size of early endosomes
Ramanathan, Ye
Cell Res. (2012) 22 (2), 346-59 DOI: 10.1038/cr.2011.80
Cited by 103
AI Summary: OriGene’s full-length human EEA1 protein was used in this study to investigate the interaction between p97 ATPase and EEA1, helping to elucidate p97’s role in regulating early endosome size.
Snippet: Compared with the gene-silencing approach, chemical inhibitors can rapidly shut down protein function and thus allow observation of the immediate effect associated with loss of protein function. ... Full-length human EEA1 was purchased (OriGene).
Showing 1-2 of 2 papers.
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