PGP (NM_001042371) Human Mass Spec Standard

CAT#: PH314470

PGP MS Standard C13 and N15-labeled recombinant protein (NP_001035830)

Product Images

Coomassie blue staining of purified PGP protein (Cat# TP314470). The protein was produced from HEK293T cells transfected with PGP cDNA clone (Cat# RC214470) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC214470
• Predicted MW: 33.8 kDa
• Protein Sequence:
  Sequence Link

  >RC214470 representing NM_001042371
  Red=Cloning site Green=Tags(s)
  
  MAAAEAGGDDARCVRLSAERAQALLADVDTLLFDCDGVLWRGETAVPGAPEALRALRARGKRLGFITNNS
  SKTRAAYAEKLRRLGFGGPAGPGASLEVFGTAYCTALYLRQRLAGAPAPKAYVLGSPALAAELEAVGVAS
  VGVGPEPLQGEGPGDWLHAPLEPDVRAVVVGFDPHFSYMKLTKALRYLQQPGCLLVGTNMDNRLPLENGR
  FIAGTGCLVRAVEMAAQRQADIIGKPSRFIFDCVSQEYGINPERTVMVGDRLDTDILLGATCGLKTILTL
  TGVSTLGDVKNNQESDCVSKKKMVPDFYVDSIADLLPALQG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_001035830
• RefSeq Size: 1041
• RefSeq ORF: 963
• Synonyms: AUM; G3PP; PGPase
• Locus ID: 283871
• UniProt ID: A6NDG6
• Cytogenetics: 16p13.3
• Summary: Glycerol-3-phosphate phosphatase hydrolyzing glycerol-3-phosphate into glycerol. Thereby, regulates the cellular levels of glycerol-3-phosphate a metabolic intermediate of glucose, lipid and energy metabolism. Was also shown to have a 2-phosphoglycolate phosphatase activity and a tyrosine-protein phosphatase activity. However, their physiological relevance is unclear (PubMed:26755581). In vitro, has also a phosphatase activity toward ADP, ATP, GDP and GTP (By similarity).[UniProtKB/Swiss-Prot Function]
• Protein Pathways: Glyoxylate and dicarboxylate metabolism, Metabolic pathways